| 紫玉兰愈伤组织的诱导及胚状体获得试验研究 |
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| 引用本文： | 罗在柒,刘兰,周大刚.紫玉兰愈伤组织的诱导及胚状体获得试验研究[J].现代农业科技,2010(20):221+223. |
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| 作者姓名： | 罗在柒 刘兰 周大刚 |
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| 作者单位： | 贵州省林业科学研究院;赤水市第一中学; |
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| 摘 要： | 用正交统计方法,以MS为基本培养基,调节2,4-D、6-BA和NAA之间的配比,对紫玉兰的雌蕊、雄蕊、花被、花萼和带腋芽枝条等组织进行愈伤组织诱导,试验结果表明:雌蕊在MS+2,4-D 4.00 mg/L+6-BA 1.00 mg/L+NAA 4.00 mg/L能够产生浅黄绿色丝状愈伤组织;花萼在MS+2,4-D 4.00 mg/L+6-BA 1.00 mg/L+NAA 2.00 mg/L能够产生淡黄色块状愈伤组织;腋芽在MS+2,4-D 4.00 mg/L+6-BA 2.00mg/L+NAA 4.00 mg/L能够诱导产生浅绿色团状愈伤组织,该组织经转接培养后产生胚状体。
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| 关 键 词： | 愈伤组织 胚状体 诱导 紫玉兰 |
Experimental Research on Callus Induction and Embryoid Body Formation of Magnolia liliflora Desr. |
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| Authors: | LUO Zai-qi LIU Lan ZHOU Da-gang |
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| Institution: | LUO Zai-qi 1 LIU Lan 1 ZHOU Da-gang 2(1Guizhou Academy of Forestry Sciences,Guiyang Guizhou 550005,2 No.1 Middle School in Chishui City) |
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| Abstract: | By orthogonal statistical using MS as the basic medium and regulating the rate of 2,4-D,6-BA and NAA,the callus of Magnolia liliflora Desr.’s gynoecium,androecium,perianth,calyx and axillary bud in vitro were induced.Results showed that MS+2,4-D 4.00 mg/L+6-BA 1.00 mg/L+NAA 4.00 mg/L adapted to induce the light green yellow filamertous callus from gynoecium;MS+2,4-D 4.00 mg/L+6-BA 1.00 mg/L +NAA 2.00 mg/L adapted to induce the buff tuberous callus from calyx;MS+2,4-D 4.00 mg/L+6-BA 2.00 mg/L+NAA 4.00 mg/L adaptd to induce the light green globall callus from axillary bud which organized embryoid body after inoculating. |
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| Keywords: | callus embryoid body induction Magnolia liliflora Desr |
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