| 环氧合酶-1和环氧合酶-2在兔早期妊娠子宫内膜及早期胚胎中的表达 |
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| 引用本文： | 滕春波,徐立滨,李世杰,杨增明.环氧合酶-1和环氧合酶-2在兔早期妊娠子宫内膜及早期胚胎中的表达[J].中国兽医学报,2003,23(5):499-502. |
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| 作者姓名： | 滕春波 徐立滨 李世杰 杨增明 |
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| 作者单位： | 东北农业大学,生命科学学院,黑龙江,哈尔滨,150030 |
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| 基金项目： | 国家自然科学基金资助项目 (3 0 2 70 163 ) |
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| 摘 要： | 环氧合酶(cyclooxygenase，Cox)是由花生四烯酸合成前列腺素的限速酶，而前列腺素在哺乳动物的生殖过程中起着很重要的作用。本试验采用RT—PCR方法对环氧合酶-1(Cox-1)及环氧舍酶-2(Cox-2)在早期妊娠兔子宫内膜中的表达进行了半定量研究和在早期胚胎中的表达定性研究。结果表明，Cox-1在早期妊娠兔子宫内膜中表达较平稳，呈组成型表达；在早期胚胎中各个时期均可以检测到Cox-1mRNA，表明它在早期胚胎发育过程中起作用。Cox-2在早期妊娠子宫内膜中的表达水平有很大差异，妊娠第l天表达水平较高，从第2天开始表达下降，到第6天表达又明显增加，第8天达到最高。Cox-2在囊胚之前的各个时期胚胎均未检测到，而在扩展胚泡和孵出胚泡中表达，Cox-2的时期特异性表达可能与兔胚泡孵出及胚胎着床等过程相关。
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| 关 键 词： | 环氧合酶-1 环氧合酶-2 兔 早期妊娠 子宫内膜 早期胚胎 表达 前列腺素 限速酶 生殖 胚胎着床 |
| 文章编号： | 1005-4545(2003)05-0499-04 |
| 修稿时间： | 2002年6月20日 |
Expression of Cyclooxygenase-1 and Cyclooxygenase-2 in Rabbit Endometrium during Early Pregnancy and in Early Stage Embryos |
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| TENG Chun bo,XU Li bin,LI Shi jie,YANG Zeng ming.Expression of Cyclooxygenase-1 and Cyclooxygenase-2 in Rabbit Endometrium during Early Pregnancy and in Early Stage Embryos[J].Chinese Journal of Veterinary Science,2003,23(5):499-502. |
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| Authors: | TENG Chun bo XU Li bin LI Shi jie YANG Zeng ming |
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| Abstract: | Cyclooxygenase is a rate limiting enzyme that converts arachidonic acid to prostaglandin.It exists as two isoforms:cyclooxygenase 1(Cox 1) and cyclooxygenase 2(Cox 2),which are encoded by separate genes.RT PCR method is used to study the expression of Cox 1 and Cox 2 in rabbit endometrium during early pregnancy by semi quantitative method and to study their expression in early stage embryos by qualitative method.Cox 1 is constitutively expressed in the endometrium during early pregnancy and it can be detected in every stage of early embryos.There is obvious change for Cox 2 expression in the endometrium.A high level of Cox 2 expression is detected on day 1 of pregnancy,it decreases from day 2 to 5,increases again from day 6,and reaches its highest level on day 8.Cox 2 can only be detected in expand blastocyst and hatched blastocyst.The stage specific expression of Cox 2 may be related to embryo hatching and implantation. |
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| Keywords: | cyclooxygenase rabbit early pregnancy uterus embryo implantation |
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