| 重组N蛋白抗原检测PRRSV抗体的ELISA研制——Ⅲ.ELISA试剂盒保存条件 |
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| 引用本文： | 吴延功,徐天刚,王志亮,张喜悦,王君玮,宋翠平,刘佩兰,徐培莲,宋芳.重组N蛋白抗原检测PRRSV抗体的ELISA研制——Ⅲ.ELISA试剂盒保存条件[J].中国兽医学报,2006,26(4):347-349. |
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| 作者姓名： | 吴延功 徐天刚 王志亮 张喜悦 王君玮 宋翠平 刘佩兰 徐培莲 宋芳 |
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| 作者单位： | 农业部动物检疫所,山东,青岛,266032 |
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| 基金项目： | 国家重大科技专项基金;国家农业科技成果转化基金 |
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| 摘 要： | 对应用重组N蛋白抗原检测猪繁殖与呼吸综合征病毒（PRRSV）抗体的ELISA诊断方法的主要成分（抗原包被板、酶标二抗、阳性血清、阴性血清）及组装成的试剂盒的保存条件进行了摸索。分别用5种方法时抗原包被板处理，结果表明用真空包装机抽真空后充入氮气法处理效果最好；酶标二抗在酶标抗体保存液中保存，能很好地保持ELISA测定结果的稳定性。时其他试剂盒组成成分的保存期分别测定后，组装成试剂盒，测定试剂盒的保存期为12个月。
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| 关 键 词： | 猪繁殖与呼吸综合征病毒 试剂盒 保存期 |
| 文章编号： | 1005-4545(2006)04-0347-03 |
| 收稿时间： | 2004-11-16 |
| 修稿时间： | 2004年11月16 |
Development of ELISA Kit Using Recombinant Nucleoprotein as Antigen of PRRS Virus Ⅲ.The Storage Conditions of the ELISA Kits |
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| WU Yan-gong,XU Tian-gang,WANG Zhi-liang,ZHANG Xi-yue,WANG Jun-wei,SONG Cui-ping,LIU Pei-lan,XU Pei-lian,SONG Fang.Development of ELISA Kit Using Recombinant Nucleoprotein as Antigen of PRRS Virus Ⅲ.The Storage Conditions of the ELISA Kits[J].Chinese Journal of Veterinary Science,2006,26(4):347-349. |
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| Authors: | WU Yan-gong XU Tian-gang WANG Zhi-liang ZHANG Xi-yue WANG Jun-wei SONG Cui-ping LIU Pei-lan XU Pei-lian SONG Fang |
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| Institution: | National Animal Quarantine Institute,Ministry of Agriculture, Qingdao ,Shandong 266032 ,China |
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| Abstract: | In spite of many factors influence on the results of ELISA kits,the storage condition of the kits is the most important one. The storage conditions of the kit include its components ,e. g. the plates coated by recombinant N protein,diluted HRPO rabbit anti-porcine conjugate,the control positive/negative serum el al,were explored. The plates were dealt with five different methods ,with the full of nitrogen postvaccum to be the best for reserving the capability of the kits. It was shown that the kit could be stored for 12 months at 4 C. An inexpensive,rapid ,specific diagnostic assay has been developed ,which provides an available technique for the diagnosis and serological survey of PRRS, and a good fundament for the further commercial development of the kit. |
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| Keywords: | ELISA |
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