Study on the Obtaining of Transgenic Wheat with GNA Alien Gene by Biolistic Particle |
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| Authors: | XU Qiong-Fang LI Lian-cheng CHEN Xiao Tian fang MA You-Zhi YE Xing-guo ZHANG Zeng-Yan XU Hui-jun XIN Zhi-yong |
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| Institution: | Key Laboratory of Agricultural Ministry for Crop Genetics and Breeding, Institute of Crop Breeding and Cultivation, Chinese Academy of Agricultural Sciences, Beijing 100081 |
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| Abstract: | The immature embryos of wheat plants, cv. Jing 411, 12 - 14 days after pollination, were cultured on SD2 medium for callus induction. After 10 days culture, 800 wheat calli were bombarded by biolistic particle coated with theDNA of plasmid pBI121-2 harboring both Galanthus nivalis agglutinin gene and bar gene. 67 green plants were finally regenerated from the bombardment calli on selection medium containing 4mg/L Basta. The results of bioassay by both inoculating wheat aphids onto the plants and applying Basta solution of 50 mg/L and 75 mg/L onto the wheat leaves in the field, and the molecular analysis, such as PCR and Southern blotting, indicated that 8 T2 plants contaning the target genes were obtained. |
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| Keywords: | Galanthus nivalis agglutinin bar gene Particle bombardment Transgenic wheat |
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