| 沙田柚多倍体的获得与基因组原位杂交（GISH）分析 |
| |
| 引用本文： | 向素琼,梁国鲁,李晓林,汪卫星,郭启高,何桥,陈瑶.沙田柚多倍体的获得与基因组原位杂交（GISH）分析[J].中国农业科学,2008,41(6):1749-1754. |
| |
| 作者姓名： | 向素琼 梁国鲁 李晓林 汪卫星 郭启高 何桥 陈瑶 |
| |
| 作者单位： | [1]西南大学园艺园林学院,重庆400716 [2]中国农业科学院柑橘研究所,重庆400712 |
| |
| 基金项目： | 国家自然科学基金
,
重庆市教委资助项目 |
| |
| 摘 要： | 【目的】沙田柚是中国特有的柚类名优品种，但种子多，一般100粒左右。为创新三倍体无核品种积累育种材料，本研究通过实生筛选获得不同倍性沙田柚新种质，同时运用基因组原位杂交（GISH）技术分析天然与人工四倍体新种质的染色体组组成。【方法】随机采集沙田柚自然授粉果实，萌发种子检测其染色体数目获得倍性变异植株；以2x母株gDNA为探针，同获得的4x植株中期染色体杂交进行GISH分析。【结果】从6 000粒沙田柚种子中共获得三倍体5株，四倍体9株；对沙田柚天然与人工四倍体新种质的基因组原位杂交（GISH）分析表明，天然四倍体中有7株为异源四倍体，2株为同源四倍体，秋水仙碱诱导获得的人工四倍体4株均为同源四倍体；初步观察显示；与二倍体相比，四倍体植株生长缓慢，树冠较小，枝短而密生，叶片浓绿，宽度变宽，叶形指数减小，叶片厚度增加明显。【结论】多倍体单胚柚新种质的获得为进一步选育无核品种奠定了基础，同时GISH分析证实了沙田柚雌性未减数配子的存在。
|
| 关 键 词： | 沙田柚 多倍体 基因组原位杂交 未减数配子 |
| 收稿时间： | 2007-3-20 |
Obtaining Polyploids and Genome Analysis of Tetraploids by GISH in Citrus grandis cv. Shatianyou |
| |
| XIANG Su-qiong,LIANG Guo-lu,LI Xiao-lin,WANG Wei-xing,GUO Qi-gao,HE Qiao,CHEN Yao.Obtaining Polyploids and Genome Analysis of Tetraploids by GISH in Citrus grandis cv. Shatianyou[J].Scientia Agricultura Sinica,2008,41(6):1749-1754. |
| |
| Authors: | XIANG Su-qiong LIANG Guo-lu LI Xiao-lin WANG Wei-xing GUO Qi-gao HE Qiao CHEN Yao |
| |
| Abstract: | Abstract: 【Objective】Shatianyou (Citrus grandis Osbeck cv. Shatianyou) is a peculiar excellent pummelo cultivar in China,but it has many seeds, about 100. The aim of the experiment was to obtain different ploidy new germplasm for seedless triploid breeding by means of screening seedlings, and to analyze genome constitution of natural and artificial tetraploids by Genome in situ hybridization(GISH).【Method】Collecting randomly open-pollinated seeds, then ploidy variation seedlings were gained from them by chromosome counting; In order to analyze genome constitution of tetraploids, the labeled total gDNA of diploid as probe was hybridized to tetraploid metaphase chromosomes by GISH.【Result】5 triploids and 9 tetraploids were found from 6000 seeds. GISH analysis of tetraploids showed that there were 7 allotetraploid and 2 autotetraploid in natural tetraploids, while 4 artificial tetraploids from colchicine induction were all autotetraploid.【Conclusion】These monoembryonic polyploids would be for seedless breeding. GISH result proved existence of unreduced eggs in Citrus grandis cv. Shatianyou. |
| |
| Keywords: | Citrus grandis cv Shatianyou polyploidy Genome in situ hybridization (GISH unreduced gamete |
| 本文献已被 维普 万方数据 等数据库收录！ |
| 点击此处可从《中国农业科学》浏览原始摘要信息 |
| 点击此处可从《中国农业科学》下载免费的PDF全文 |
|
