Cryopreservation of strip spawned sperm using non‐programmable freezing technique in the blue mussel Mytilus galloprovincialis |
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| Authors: | Shiwen Liu Tong Xu Qing Liu Xiaoxu Li |
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| Institution: | 1. Dalian Ocean University, Dalian, China;2. South Australian Research and Development Institute, West Beach, South Australia, Australia;3. Marine Innovation Southern Australia, West Beach, South Australia, Australia |
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| Abstract: | In this study, a non‐programmable freezing technique has been developed with the strip spawned blue mussel (Mytilus galloprovincialis) sperm. The key parameters optimized including (1) cryoprotectant agents (CPAs); (2) cooling temperature; (3) thawing temperature; (4) sugar and amino acid supplementation and (5) sperm to egg ratio. The fertilization rate and/or integrity of plasma membrane and acrosome were used as sperm quality assessment indicators. The highest post‐thaw sperm fertilization rate of 95% was achieved, when sperm were cryopreservated in 8% dimethyl sulfoxide at 7.8 cm above the liquid nitrogen surface and thawed in a 60°C seawater bath. The addition of glucose, sucrose or trehalose in dimethyl sulfoxide did not, whereas 0.8% glycine did significantly improve the post‐thaw sperm fertilization rates. The fluorescent evaluation has demonstrated that the addition of glycine significantly improved the post‐thaw sperm acrosome integrity, revealing a positive role of glycine in the improvement of post‐thaw sperm quality in blue mussels. |
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| Keywords: | cryopreservation blue mussel
Mytilus galloprovincialis
strip spawned sperm non‐programmable freezing technique glycine |
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