Effect of chemical and physical treatments on the inactivation of Macrobrachium rosenbergii nodavirus and extra small virus |
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| Authors: | Mani Ravi A Sait Sahul Hameed |
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| Institution: | 1. Centre for Ocean Research, Sathyabama University, Chennai, Tamil Nadu, India;2. OIE Reference Laboratory for WTD, Department of Zoology, C. Abdul Hakeem College, Vellore, Tamil Nadu, India |
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| Abstract: | White tail disease (WTD) is found to cause immense economic losses in hatcheries, with mortalities often reaching 100% within 4 or 5 days. The pathogenic agents have been identified as Macrobrachium rosenbergii nodavirus (MrNV) associated with extra small virus (XSV), which are 27 and 15 nm in diameter respectively. The effects of some chemical disinfectants hydrogen ions (pH), heat and ultraviolet (UV) irradiation on the inactivation of MrNV and XSV were investigated. The viral inoculum exposed to UV irradiation for a period of 5 min and more was totally inactivated and failed to cause mortality in postlarvae of prawn. The viruses were totally inactivated by this high pH (8.5, 9 and 10). The viral suspension treated with sodium hypochloride, formalin, Benzalkonium chloride and Benzethonium chloride at the concentration of 200 ppm caused 100% mortality in postlarvae of prawn. Iodine was found to be effective to inactivate MrNV and XSV at the concentration of 100 ppm or more, whereas the viral suspension treated with iodine at the concentration of 50 ppm or less caused mortality in postlarvae. The infected postlarvae in treated and positive control groups showed positive by RT‐PCR for these viruses. |
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| Keywords: | white tail disease
Mr
NV
XSV
treatment chemical physical |
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