| 不同软籽品种石榴抗寒性综合评价 |
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| 引用本文： | 姚方,王宁,曹尚银,马贯羊,司守霞,姚海雷.不同软籽品种石榴抗寒性综合评价[J].福建林学院学报,2016(3):373-379. |
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| 作者姓名： | 姚方 王宁 曹尚银 马贯羊 司守霞 姚海雷 |
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| 作者单位： | 1. 河南林业职业学院,河南 洛阳,471002;2. 河南科技大学林学院,河南 洛阳,471003;3. 中国农业科学院郑州果树研究所,河南 郑州,450009;4. 洛阳农林科学院,河南 洛阳,471000 |
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| 基金项目： | 国家林业局“948”项目“软籽石榴品种资源及集约培育技术引进”(2012－4－55)；河南省教育厅科学技术研究重点项目“以色列软籽石榴的引种适应性评价及快繁技术研究”(14B210029)；河南省科技发展计划项目“以色列软籽石榴引种、繁育及配套栽培技术研究”(142102110169)。 |
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| 摘 要： | 结合低温胁迫过程生理生化指标的变化,对项目组引种的5个以色列软籽品种石榴进行抗寒性评价。以突尼斯及以色列软籽品种石榴的1年生枝条为试材,采用人工模拟低温(4、0、－5、－10、－15℃)进行低温处理,对其相对电导率、可溶性糖、脯氨酸、可溶性蛋白质和丙二醛( MDA)含量以及过氧化物歧化酶( SOD)活性进行测定,并结合主成分分析和隶属函数法对8个品种石榴的抗寒性进行综合评价。结果表明,随着胁迫温度的降低,8个品种石榴枝条的相对电导率呈“S”型曲线变化, SOD活性,可溶性糖、脯氨酸及可溶性蛋白质含量均呈先升后降的变化趋势,而MDA含量呈先降后升的变化。8个品种石榴抗寒性为‘豫大籽’>‘以色列3号’>‘突尼斯’>‘以色列1号’>‘以色列2号’>‘中农红’>‘以色列M’>‘以色列Y’。
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| 关 键 词： | 软籽石榴 抗寒性 生理生化指标 |
Comprehensive evaluation on cold resistance of different pomegranate cultivars |
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| Abstract: | According to the physiological and biochemical indices in the low temperature stress process, the cold resistance of 5 Israeli soft-seeded pomegranate cultivars was comprehensively evaluated to offer a reference for the introduction and cultivation of those fruit trees in the north regions of China. Annual shoots of 8 Tunisian and Israeli soft-seeded pomegranate cultivars were used as the experimental materials to observe the effect of low temperature stress on the young sprout, relative conductivity, superoxide dismutase ( SOD ) activity and the content of proline, soluble protein, soluble sugar, malondialdehyde ( MDA ) under the low temperature stress ( 4, 0, -5, -10 and -15 ℃) were measured, and cold resistance was comprehensively evaluated by principal components analysis and membership function. Along with the temperature decreasing, the relative conductivity in the shoots of 8 soft-seeded pomegranate cultivars changed in an S-curve, and the content of soluble protein, proline, soluble sugar, and SOD activity tended to increase, followed by decreasing, but the content of malondialdehyde decreased at the beginning and then increased. The cold resistance of 8 soft-seeded pomegranate cultivars was as follows: ‘Yudazi ’ > ‘Israel 3 ’ > ‘Tunisia ’ >‘Israel 2’> ‘Israel 1’ > ‘Zhongnonghong’ > ‘Israel M’ > ‘Israel Y’ . |
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| Keywords: | soft-seeded pomegranate cold resistance physiological and biochemical indexes |
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