| 珍珠黄杨叶片的蛋白质提取方法探讨 |
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| 引用本文： | 王明娟,季孔庶.珍珠黄杨叶片的蛋白质提取方法探讨[J].广西农业生物科学,2009(1):105-108. |
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| 作者姓名： | 王明娟 季孔庶 |
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| 作者单位： | 林木遗传与生物技术省部共建教育部重点实验室;南京林业大学; |
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| 基金项目： | 十一五国家科技支撑项目(2006BAD01A1403)资助 |
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| 摘 要： | 蛋白质样品制备是双向电泳的核心。为了找到一种适合提取珍珠黄杨叶片蛋白质的方法，本文以该树种扦插苗的叶片为材料，用TCA_丙酮沉淀法、Tris-饱和酚法和2-D Clean-Up Kit提取蛋白质，并进行双向凝胶电泳，采用银染法进行检测。结果表明，TCA-丙酮沉淀法得到的样品图谱背景模糊、拖尾；Tris-饱和酚法得到的样品图谱清晰，蛋白点饱满，无纵向或横向拖尾，但有蛋白点丢失；2-DClean-Up Kit提取的蛋白质样品得到了较好双向电泳图谱。
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| 关 键 词： | 珍珠黄杨叶片 蛋白质提取 双向电泳 |
The Study of Protein Extraction from Buxus sinica var. parvifolia Leaves |
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| Wang Mingjuan Ji Kongshu Key Laboratory of Forest Genetics & Biotechnology,Ministry of Education,Nanjing Forestry University,Nanjing.The Study of Protein Extraction from Buxus sinica var. parvifolia Leaves[J].Journal of Guangxi Agricultural and Biological Science,2009(1):105-108. |
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| Authors: | Wang Mingjuan Ji Kongshu Key Laboratory of Forest Genetics & Biotechnology Ministry of Education Nanjing Forestry University Nanjing |
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| Institution: | Wang Mingjuan Ji Kongshu Key Laboratory of Forest Genetics & Biotechnology,Ministry of Education,Nanjing Forestry University,Nanjing,210037 |
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| Abstract: | Protein sample preparation is the key of two-dimensional gel electrophoresis. In order to find a method suitable for protein extraction from leaves of Buxus sinica var. parvifolia. The leaves were collected from steckling and proteins were extracted with the methods of trichloroacetic acid (TCA)-acetone, Tris-saturated phenol and 2-D Clean-Up Kit. Then the two-dimensional gel electrophoresis (2-DE), sliver staining were used to detect the extracted proteins. The result indicated that protein pattern backgro... |
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| Keywords: | Buxus sinica var parvifolia leaves Protein extraction Two-dimensional gel electrophoresis |
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