Isolation, identification, and pathogenicity of two field strains of infectious bursal disease virus |
| |
| Authors: | A G Rosales P Villegas P D Lukert O J Fletcher M A Mohamed J Brown |
| |
| Institution: | Department of Avian Medicine, College of Veterinary Medicine, University of Geoggia, Athens 30602. |
| |
| Abstract: | Using a sentinel bird approach, two field isolates of infectious bursal disease virus (IBDV) were isolated from broiler farms in two major broiler-producing areas of the state of Georgia. These farms had a history of subclinical IBD associated with respiratory problems and poor performance. Isolates designated as U-28 and 3212 were isolated using specific-pathogen-free chicken embryos and chicken embryo bursal cells. These isolates were identified by means of agar gel precipitation and virus-neutralization tests, direct immunofluorescence, histopathology, and electron microscopy. Isolates U-28 and 3212 appear to differ in antigenicity and pathogenicity from previously known serotype I IBDV isolates. In evaluating the extent of bursal damage caused by these field isolates, an association was found between the bursa of Fabricius/body weight index, histopathology scoring of atrophy, and morphometric analysis of the total follicle area. |
| |
| Keywords: | |
|
|
