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高纯度茶色素产品的化学组成与检测
引用本文:屠幼英,梁慧玲,杨贤强,陈利燕.高纯度茶色素产品的化学组成与检测[J].茶叶,2002,28(4):191-193.
作者姓名:屠幼英  梁慧玲  杨贤强  陈利燕
作者单位:浙江大学茶学系,杭州,310029
摘    要:用高压液相色谱同步分析测定了用固定化多酚氧化酶生化反应器生产的茶色素产品的茶黄素和儿茶素的含量和组成。产品的茶黄素总量为78.94%,其中TF5.89%、TF3G14.54%、TF3’G11.17%、TFDG47.34%。产品中残留的儿茶素为13.83%,其余7.23%为茶红素等成分。

关 键 词:纯度  茶色素  化学组成  固定化多酚氧化酶  茶黄素  儿茶素
文章编号:0577-8921(2002)04-191-03
修稿时间:2002年9月4日

The detection and composition of purified tea pigment
Tu Youying,Liang Huiling,Yang Xianqiang,Chen LiyanCDept of Tea Science,Zhejiang University,Hangzhou.The detection and composition of purified tea pigment[J].Journal of Tea,2002,28(4):191-193.
Authors:Tu Youying  Liang Huiling  Yang Xianqiang  Chen LiyanCDept of Tea Science  Zhejiang University  Hangzhou
Institution:Tu Youying,Liang Huiling,Yang Xianqiang,Chen LiyanCDept of Tea Science,Zhejiang University,Hangzhou 310029)
Abstract:Abstract The composition and content of tea catechins and theaflavins in the tea pigment obtained from tea polyphenols catalyzed with immobilized polyphenol oxidase isolated were measured simultaneously using Shimadzu LC-10A high-pressure liquid chromatograph (HPLC). The tea pigment contained 78. 94% of theaflavins which consisted of theaflavin (TF) 5. 89 %, theaflavin-3-gallate (TF3G) 14.54%, theaflavin-3'-gallate (TF3'G) 11.14%, and theaflavin-3, 3'-digallate (TF3,3'G) 47. 34%. The residual catechins in the pigment was 13. 83%, and the other 7. 23% was thearubigins and some other compounds.
Keywords:Immobilized polyphenol oxidase  Theaflavin  Catechin  Detection
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