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环境因子对鲍和牡蛎精子运动能力及受精率的影响
引用本文:李霞,刘淑范,康蕾,徐丹霞.环境因子对鲍和牡蛎精子运动能力及受精率的影响[J].大连水产学院学报,2002,17(1):25-30.
作者姓名:李霞  刘淑范  康蕾  徐丹霞
作者单位:大连水产学院,养殖系,辽宁,大连,116023
基金项目:国家自然科学基金资助项目 (3 9870 60 0 )
摘    要:研究了环境因子如K^ 、Na^ 、Ca^2 、Mg^2 、Cl^-、SO4^2-以及蔗糖、pH、氨海水对皱纹盘鲍(Haliotis discus Hannai)和太平洋牡蛎(Creastrea gigas)精子运动及受精率的影响。精子在0.4-0.5mol/L的NaCl、KCl溶液中运动能力为1005,但受精能力较差或完全不受精。在替代海水中无Ca^2 的情况下,牡蛎不受精,但鲍有较低的受精能力。Mg^2 作用不明显。在0.5-0.6mol/L蔗糖溶液中鲍精子能运动,且有较低的受精能力,但牡蛎精子完全不能运动和受精。氨海水对精子运动有一定的刺激作用。在pH为6.0-8.0时精子运动能力较强,但受精的pH适宜范围为7.0-8.0,同时鲍和牡蛎在精子运动和受精方面有明显差异。

关 键 词:环境因子  精子运动  受精  皱纹盘鲍  太平洋牡蛎  贝类
文章编号:1000-9957(2002)01-0025-06

The influence of environmental factors on sperm motion and fertilization of the abalone and oyster
LI Xia,\ LIU Shu fan,\ KANG Lei,\ XU Dan xia.The influence of environmental factors on sperm motion and fertilization of the abalone and oyster[J].Journal of Dalian Fisheries University,2002,17(1):25-30.
Authors:LI Xia  \ LIU Shu fan  \ KANG Lei  \ XU Dan xia
Abstract:Influence of environmental factors, including K\++,Na\++,Ca\+\{2+\},Mg\+\{2+\},Cl\+-,SO\+\{2-\}\-4, sucrose (C\-\{12\}H\-\{22\}O\-\{11\}),pH and NH\++\-4 seawater on sperm motion and fertilization of abalone (\%Haliotis discus\% Hannai) and oyster (\%Creastrea gigas\%) was studied. The rate of sperm motion was 100% at NaCl or KCl concentrations of 0.4-0.5 mol/L,but their fertilities were lower or not. Exposure to seawater without Ca\+\{2+\}, the ferlilization of oyster was zero and abalone was little. There was no significance in fertility in Mg\+\{2+\}. The sperm of abalone did not move but fertilizate in a lower rate. At sucrose (C\-\{12\}H\-\{22\}O\-\{11\}) concentrations of 0.5-0.7 \{mol/L\}, however the sperm of oyster did not completely fertilize. The NH\++\-4 seawater could stimulate the sperm to move to a certain extent. The sperm moved mostly in the range of pH=6.0-8.0,and the fertilization was normal within pH=7.0-8.0.
Keywords:environmental factor  sperm motion  fertilization  \%Haliotis discus\%  \%Creastrea gigas\%
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