| 河北省2010年猪繁殖与呼吸综合征病毒的遗传变异分析 |
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| 引用本文： | 王金凤,王占峰,韩庆安,孙继国.河北省2010年猪繁殖与呼吸综合征病毒的遗传变异分析[J].广东畜牧兽医科技,2011,36(4):32-36. |
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| 作者姓名： | 王金凤 王占峰 韩庆安 孙继国 |
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| 作者单位： | 河北农业大学动物科技学院;北京海淀中海动物保健科技公司;河北省动物疫病预防控制中心; |
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| 摘 要： | 为了解2010年河北省猪繁殖与呼吸综合征病毒变异趋势，用RT—PCR方法对2010年河北省发病猪场的病料进行PRRSV Nsp2和0RF5基因扩增、克隆和测序，利用DNAStar软件对所测序列进行分析。结果显示，扩增的Nsp2基因氨基酸序列相似性为91．4％～98．8％，与GenBank中参考毒株的氨基酸序列相似性为6...
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| 关 键 词： | 猪繁殖与呼吸综合征病毒 Nsp2基因 ORF5基因 变异分析 |
Variation analysis of porcine reproductive and respiratory syndrome virus strains isolated from Hebei province in 2010 |
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| Wang Jinfeng,Wang Zhanfeng,Han Qingan,Sun Jiguo.Variation analysis of porcine reproductive and respiratory syndrome virus strains isolated from Hebei province in 2010[J].Guangdong Journal of Animal and Veterinary Science,2011,36(4):32-36. |
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| Authors: | Wang Jinfeng Wang Zhanfeng Han Qingan Sun Jiguo |
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| Institution: | Wang Jinfeng1,Wang Zhanfeng3,Han Qingan2,Sun Jiguo1*(1.College of Animal Science and Technology,Agricultural University of Hebei,Baoding 071001,China,2.Animal Disease Prevention and Control Center of Hebei,Baoding 071000,3.Beijing Haidian Zhonghai Animal Health Science Technology Company,Beijing 100086,China) |
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| Abstract: | To investigate the variation of porcine reproductive and respiratory syndrome virus in Hebei province, samples collected from six intensive pig farms suffered from PRRSV in 2010 were used for RT-PCR. The ORF5 and Nsp2 genes were subjected to PCR amplifica |
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| Keywords: | Highlypathogenic PRRSV Nsp2 gene ORF5 gene Variation analysis |
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