| 云南盘(绚)人工繁殖及胚胎发育初步观察 |
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| 引用本文： | 赵健蓉,解崇友,蔡瑞钰,黄静,苏胜齐,王志坚.云南盘(绚)人工繁殖及胚胎发育初步观察[J].南方水产科学,2017(5):124-128. |
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| 作者姓名： | 赵健蓉 解崇友 蔡瑞钰 黄静 苏胜齐 王志坚 |
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| 作者单位： | 1. 西南大学生命科学学院,淡水鱼类资源与生殖发育教育部重点实验室,水产科学重庆市市级重点实验室,重庆400715;2. 西南大学动物科技学院,重庆,400715 |
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| 基金项目： | 重庆市科委项目(cstc2016shmsx80089) |
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| 摘 要： | 采用催产素绒毛膜促性腺激素(human chorionic gonadotrophin,HCG)、促黄体素释放激素A2(luteinizing hormone releasing hormone A2,LHRH-A2)和地欧酮(domperidone,DOM)对性腺发育良好的野生云南盘(绚)(Discogobio yunnanensis)进行人工催产,干法授精.利用体视镜观察早期胚胎发育过程,并详细记录了各发育阶段的形态特征及发育时序.结果显示,云南盘(绚)成熟卵圆形、黄绿色、弱粘性,卵径平均2.1 mm.受精后卵迅速吸水膨胀,卵膜外径平均3.29 mm.在水温17 ～19℃条件下,胚胎发育历时168 h 30 min,胚胎发育过程可划分为受精卵、卵裂、囊胚、原肠胚、神经胚和器官发生6个阶段,共27个分期.初孵仔鱼平均体全长为7.16 mm,平均体质量为0.012 g,初孵仔鱼口凹形成,鳃循环明显,泄殖孔处可见输尿管,残余卵黄囊较大.
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| 关 键 词： | 云南盘(绚) 胚胎发育 人工催产 |
A preliminary study on artificial propagation and embryonic development of Discogobio yunnanensis |
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| ZHAO Jianrong,XIE Chongyou,CAI Ruiyu,HUANG Jing,SU Shengqi,WANG Zhijian.A preliminary study on artificial propagation and embryonic development of Discogobio yunnanensis[J].South China Fisheries Science,2017(5):124-128. |
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| Authors: | ZHAO Jianrong XIE Chongyou CAI Ruiyu HUANG Jing SU Shengqi WANG Zhijian |
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| Abstract: | Discogobio yunnanensis with good gonadal development were investigated by artificial spawning,dry fertilization and fertilized by dry artificial insemination,being injected with mixture of human chorionic gonadotropin (HCG),luteinizing hormone releasing hormone A2 (LHRH-A2) and domperidone (DOM).The morphological traits and developmental time of embryonic developmental stages were recorded in great details by stereomicroscope.Results show that the yellow-green eggs of D.yunnanensis were spherical,pe lagic,slightly adhesive with an average diameter of 2.1 mm,and 3.29 mm after absorbing water.The time for embryonic development was 168 h 30 min when the water temperature was 17 ～ 19 ℃.The embryonic development of D.yunnanensis can be divided into 27 development phases and six stages,including fertilized eggs period,cleavage period,blastula period,gastrula period,neurula and organogenetic period.The average length and weight of the newly hatched fry were 7.16 mm and 0.012 g,respectively.The newly hatched larvae were almost developed completely,with a bigger yolk sac,an obvious branchial circulation,a rudiment of mouth and a ureter near the cloacal orifice. |
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| Keywords: | Discogobio yunnanensis embryonic development artificial propagation |
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