| 草鱼成纤维细胞gcngr1基因的表达分析 |
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| 引用本文： | 焦真真,田园园,孙成飞,董浚键,姜鹏,叶星.草鱼成纤维细胞gcngr1基因的表达分析[J].南方水产科学,2017(5):55-62. |
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| 作者姓名： | 焦真真 田园园 孙成飞 董浚键 姜鹏 叶星 |
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| 作者单位： | 1. 中国水产科学研究院珠江水产研究所,农业部热带亚热带水产资源利用与养殖重点实验室,广东广州510380;上海海洋大学水产与生命学院,上海201306;2. 中国水产科学研究院珠江水产研究所,农业部热带亚热带水产资源利用与养殖重点实验室,广东广州510380 |
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| 基金项目： | 中国水产科学研究院中央级公益性科研院所基本科研业务费专项资金资助(2016HY-ZC0602)，广东省级鱼病防治专项资金项目“草鱼病毒特异性疫苗研究与开发” |
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| 摘 要： | Ngr1(nogo-66 receptor)是在哺乳类上发现的一种神经元受体,可调节轴突的可塑性并抑制损伤中枢神经系统(CNS)的再生,最新研究还发现它是哺乳动物呼肠孤病毒(mammalian reovirus)的神经受体。草鱼呼肠孤病毒(grass carp reovirus,GCRV)可引发草鱼(Ctenopharyngodon idellus)出血病导致高死亡率,开展GCRV受体相关研究可有助于了解病毒的致病机制。该研究在草鱼吻端成纤维细胞(PSF)中克隆到草鱼ngr1 c DNA序列(下文简称为gcngr1),发现其与哺乳动物呼肠孤病毒神经受体基因Ngr1有相似的结构序列;采用q RT-PCR方法检测该基因在PSF细胞的表达情况,结果显示受草鱼呼肠孤病毒(GCRV-GD108株)感染后gcngr1 m RNA的表达量显著上升,与病毒的增殖趋势基本一致;病毒经病毒抗体孵育后再感染PSF细胞,细胞中病毒的增殖水平下降,gcngr1m RNA的表达量也显著下降。该研究结果提示gcngr1与病毒的感染相关,为进一步分析gcngr1是否为GCRV神经元受体提供依据。
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| 关 键 词： | 草鱼 ngr1 基因表达 qRT-PCR 呼肠孤病毒受体 PSF细胞 |
Expression analysis of gcngr1 gene in grass carp (Ctenopharyngodon idellus) PSF cells |
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| JIAO Zhenzhen,TIAN Yuanyuan,SUN Chengfei,DONG Junjian,JIANG Peng,YE Xing.Expression analysis of gcngr1 gene in grass carp (Ctenopharyngodon idellus) PSF cells[J].South China Fisheries Science,2017(5):55-62. |
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| Authors: | JIAO Zhenzhen TIAN Yuanyuan SUN Chengfei DONG Junjian JIANG Peng YE Xing |
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| Abstract: | The Nogo-66 receptor (ngr1),expresses in neuron surface,can inhibit the regeneration of injury central nervous system (CNS) and regulate axon plasticity in mammals.Recent studies have shown that it also serves as a mammalian reovirus (MRV) neu ron receptor.Grass carp reovirus (GCRV) can invade grass carp (Ctenopharyngodon idellus) and cause high mortality,so it would be helpful to understand the mechanisms of pathogenicityis on GCRV receptor.In this study,the grass carp ngr1 (gcngr1) cDNA was cloned from grass carp snout fibroblasts cells (PSF),which shared similar sequence with MRV receptor Ngr1;qRT-PCR was performed to detect the expression of gcngr1 and virus proliferation in grass carp snout fibroblasts (PSF) cells challenged by GCRV-GD108.The gcngr1 expression in PSF cells showed a significant increase after being infected by GCRV-GD108,which was consistent with the trend of virus reproduction.In addition,GCRV-GD108 was incubated with polyclonal antibody of GCRV-GD108 firstly and then infected PSF cells.A significant decrease in virus reproduction was observed,and gcngr1 mRNA expression also decreased in PSF cells.The results show that the expression of gcngr1 is related with GCRV infection in PSF cells,which provides references for determining whether gcngr1 is a GCRV receptor or not. |
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| Keywords: | grass carp ngr1 gene expresion qRT-PCR reovirus receptor PSF cells |
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