| 瓜类细菌性果斑病菌过敏性反应和致病性(hrp)基因簇部分基因的克隆及功能分析 |
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| 引用本文： | 汪新,王卫,钱国良,王敏杰,胡白石,刘凤权.瓜类细菌性果斑病菌过敏性反应和致病性(hrp)基因簇部分基因的克隆及功能分析[J].农业生物技术学报,2011,19(1). |
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| 作者姓名： | 汪新 王卫 钱国良 王敏杰 胡白石 刘凤权 |
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| 作者单位： | 南京农业大学植物保护学院,农业部病虫监测与治理重点开放实验室,南京,210095 |
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| 基金项目： | 国家重点基础研究发展计划(973)项目(No.2009CB119202); 全国科技支疆计划项目(No.200840102-03); 公益性行业科研专项(No.201003066)资助 |
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| 摘 要： | 过敏性反应和致病性(hrp)基因存在于革兰氏阴性植物病原细菌中,决定病原细菌对寄主植物致病性和诱导非寄主及抗病植物过敏性反应(hypersensitive response,HR)。本研究从果斑菌(Acidovorax avenaesub sp.citrulli)的hrp基因簇中克隆了hpaA、hrcT、hrcC和hrpG基因,通过同源重组的方法,分别构建了其突变体。电镜观察发现,hpaA和hrpG基因突变体的鞭毛缺失且细胞形态发生显著变化,而hrcT和hrcC基因突变体的鞭毛和细胞形态未发生明显变化。在烟草(Nicotiana tabacam)和哈密瓜(Hami cantaloupe)叶片上的测定结果显示,hpaA、hrcT和hrcC的突变体均失去在烟草上的HR激发能力和在哈密瓜叶片上的致病性;hrpG在烟草上的HR激发能力和在哈密瓜上的致病性则显著减弱;进一步的生长曲线测定结果表明,hpaA、hrcT、hrcC和hrpG的突变体的定殖能力均显著下降。相应地,功能互补后突变体基本恢复至野生表型。证明瓜类细菌性果斑病菌hrp基因作为Ⅲ型分泌系统关键组份影响病原细菌对寄主植物的致病性和对非寄主及抗病植物的过敏性...
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| 关 键 词： | 瓜类细菌性果斑病菌 过敏性反应和致病性(hrp)基因 突变 致病性 |
Cloning and Functional Analysis of A cidovorax avenae subsp.citrulli Partial Hypersensitive Response and Pathogenicity(hrp)Gene Cluster |
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| Wang Xin,Wang Wei,Qian Guoliang,Wang Minjie,Hu Baishi,Liu Fengquan.Cloning and Functional Analysis of A cidovorax avenae subsp.citrulli Partial Hypersensitive Response and Pathogenicity(hrp)Gene Cluster[J].Journal of Agricultural Biotechnology,2011,19(1). |
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| Authors: | Wang Xin Wang Wei Qian Guoliang Wang Minjie Hu Baishi Liu Fengquan |
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| Institution: | Wang Xin Wang Wei Qian Guoliang Wang Minjie Hu Baishi Liu Fengquan Key Laboratory of Monitoring and Management for Plant Diseases and Insects,Ministry of Agriculture,College of Plant Protection,Nanjing Agricultural University,Nanjing 210095,China |
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| Abstract: | The hypersensitive response and pathogenicity(hrp) genes exist in Gram-negative plant pathogenic bacteria and are responsible for the pathogenicity of bacteria.They can induce hypersensitive response(HR) on non-host and resistant plants. hpaA,hrcT,hrcC and hrpG genes were cloned from the hrp gene cluster of Acidovorax avenae subsp. citrulli. Disruption mutants of strain xjl12 were successfully generated by a single cross-over event. Electron microscopy observation showed that the cells of hpaA and hrpG muta... |
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| Keywords: | Bacterial fruit blotch of melon Hypersensitive response and pathogenicity (hrp) gene Mutant Pathogenicity |
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