| 草鱼淋巴器官中的肥大细胞 |
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| 引用本文： | 许乐仁.草鱼淋巴器官中的肥大细胞[J].水产学报,2003,27(3):233-237. |
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| 作者姓名： | 许乐仁 |
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| 作者单位： | 贵州大学动物医学系,贵州,贵阳,550025 |
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| 基金项目： | 国家自然科学基金资助(No.30060065) |
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| 摘 要： | 通过组织化学与形态学研究首次在草鱼的胸腺和头肾等淋巴器官中证实了肥大细胞的存在。草鱼肥大细胞的染色性质在某些方面似乎类似于哺乳动物的粘膜肥大细胞。Carnoy氏液及阿尔新蓝分别是优良的固定剂及染料，而中性缓冲甲醛却完全阻断草鱼肥大细胞的着染。值得注意的是一些曾被用于鉴定硬骨鱼肥大细胞(或嗜酸性颗粒细胞)的组织化学技术在本研究中未能检出草鱼肥大细胞。电镜下证实，草鱼肥大细胞中含有大量特征性的胞浆颗粒，其中充满无定形颗粒状基质，基质电子密度有的较高，有的较低，但未见特殊的亚微结构。
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| 关 键 词： | 草鱼 淋巴器官 肥大细胞 组织化学 形态学 胸腺 头 肾 染色性质 Carnoy氏液 阿尔新蓝 |
| 文章编号： | 1000-0615(2003)03-0233-05 |
| 收稿时间： | 2014/3/18 0:00:00 |
| 修稿时间： | 2002年5月27日 |
Mast cells in the lymphoid organs of Ctenopharyngodon idella |
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| XU Le ren.Mast cells in the lymphoid organs of Ctenopharyngodon idella[J].Journal of Fisheries of China,2003,27(3):233-237. |
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| Authors: | XU Le ren |
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| Institution: | Department of Animal Medicine, Guizhou University, Guiyang550025, China |
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| Abstract: | Mast cells were identif ied first in lymphoid organs including thymus, head kidney and the splenic sinus
of grass f ish ( Ctenopharyngodon idella ) histochemically and morphologically . It seemed that the staining
properties of grass f ish mast cells were similar to mammalian mucosal mast cells in some aspects. Carnoy.. s fluid
and Alcian blue proved to be the good f ixative and excellent dye for grass fish mast cell, respectively , but neutral
buffered formalin ( NBF) completely blocked the staining of the cells. It was noticed that some other
histochemical methods previously used to detect mast cells, or so called eosinophilic granule cell ( EGC ) in
teleo stean fish, failed to identify grass f ish mast cells in present study. Under electron microscopy a great deal of
characteristic cytoplasmic granules were observed in the grass f ish mast cells. The granules contained amorpholous
granular matrix but electron density of the matrix from low to high. Special substructures were not observed in the
cytogranules. |
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| Keywords: | Ctenopharyngodon idella lymphoid organs mast cell histochemistry morphology |
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