| 乌克兰鳞鲤精子超低温冷冻保存方法研究 |
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| 引用本文： | 马林,李楠,郝爽,吴会民,姜巨峰,刘克明,白晓慧,刘肖莲,李春艳,尤宏争.乌克兰鳞鲤精子超低温冷冻保存方法研究[J].水产科学,2019(4):473-478. |
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| 作者姓名： | 马林 李楠 郝爽 吴会民 姜巨峰 刘克明 白晓慧 刘肖莲 李春艳 尤宏争 |
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| 作者单位： | 天津市水产研究所 |
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| 基金项目： | 天津市渔业发展服务中心青年科技项目(J2017-13青) |
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| 摘 要： | 为研究适用于乌克兰鳞鲤精子的超低温冷冻保存方法,分析比较3种稀释液Hank′s、Cortland、Freezefish冻精稀释液,精子与每种稀释液均设置3种比例(1∶1、1∶3、1∶5)]及3种体积分数为10%的抗冻剂(二甲基亚砜、1,2-丙二醇和丙三醇)对乌克兰鳞鲤精子低温(4 ℃)保存活力的影响;运用筛选出的冷冻保护液及稀释比例,分析比较3种“3步冷冻法”以及3种解冻温度(20、30、40 ℃)对乌克兰鳞鲤精子活力的影响。试验结果表明,采用Hank′s作为稀释液,10%二甲基亚砜为抗冻剂,精子与稀释液比例为1∶3,平均降温速率为12 ℃/min,解冻温度为30 ℃时,精子活力最高(>68%)。通过对稀释液、抗冻剂、稀释比例、降温速率和解冻温度的层层筛选,建立了适宜乌克兰鳞鲤精子超低温冷冻保存的方法,在其种质保护方面具有重要意义,为开展其他鱼类精子超低温冷冻保存提供参考。
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| 关 键 词： | 乌克兰鳞鲤 超低温冷冻 稀释液 抗冻剂 精子活力 |
Cryopreservation of Sperm in Ukraine Scale Carp |
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| MA Lin,LI Nan,HAO Shuang,WU Huimin,JIANG Jufeng,LIU Keming,BAI Xiaohui,LIU Xiaolian,LI Chunyan,YOU Hongzheng.Cryopreservation of Sperm in Ukraine Scale Carp[J].Fisheries Science,2019(4):473-478. |
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| Authors: | MA Lin LI Nan HAO Shuang WU Huimin JIANG Jufeng LIU Keming BAI Xiaohui LIU Xiaolian LI Chunyan YOU Hongzheng |
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| Institution: | (Tianjin Fishery Research Institute,Tianjin 300221,China) |
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| Abstract: | The motility was determined in both fresh and post-thawed sperm preserved at 4 ℃ in three extender solutions Hank′s,Cortland,and Freezefish at 3 dilution ratios (1∶1,1∶3,1∶5) and 3 cryoprotectants dimethyl sulphoxide DMSO,propylene glycol,glycerin at 10% volume fraction in Ukraine scale carp ( Cyprinus carpio ) in order to optimize the sperm cryopreservation,and the cryopreservation protocol for Ukrainian scale carp was subsequently tested with 3 “3-steps”cooling methods and 3 thawing temperature (20, 30 and 40 ℃) by using the screening cryoprotectant and dilution ratio. The optimal post-thawed motility (>68%) was observed in the sperm preserved at sperm to Hank′s ratio=1∶3 with 10% DMSO with mean 12 ℃/min cooling rate,and 30 ℃ water bath. The findings had significant advantages in germplasm protection,and provided reference for other fish sperm cryopreservation. |
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| Keywords: | Ukraine scale carp cryopreservation extender solution cryoprotectant sperm motility |
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