| 子二代中华鲟对急性低温胁迫的生理响应 |
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| 引用本文： | 管敏,张德志,王龙,班文波,张厚本,郭柏福,姜伟.子二代中华鲟对急性低温胁迫的生理响应[J].水产科学,2019(4):458-464. |
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| 作者姓名： | 管敏 张德志 王龙 班文波 张厚本 郭柏福 姜伟 |
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| 作者单位： | 中国长江三峡集团有限公司中华鲟研究所三峡工程鱼类资源保护湖北省重点实验室 |
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| 基金项目： | 国家自然科学基金青年科学基金资助项目(51309204) |
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| 摘 要： | 为探究急性低温胁迫对子二代中华鲟抗氧化、免疫及消化酶活性的影响,将健康子二代中华鲟体质量(1.72±0.33) kg]由23 ℃水体中直接转入17、11 ℃水体中,急性胁迫2 h后取样,检测各项指标。试验结果显示,血清抗氧化指标中,超氧化物歧化酶、过氧化氢酶活性随水温骤降温差增大,呈先升后降的趋势;而谷胱甘肽、丙二醛含量则呈先降后升的趋势( P >0.05);总抗氧化能力逐渐升高。肝脏抗氧化指标中,超氧化物歧化酶和过氧化氢酶活性均呈先升后降的趋势;谷胱甘肽含量逐渐下降,而丙二醛含量却显著增加( P <0.05);总抗氧化能力出现波动( P >0.05)。免疫指标中,血清和肝脏溶菌酶活性随水温骤降幅度的增大,均呈先升后降的趋势,而免疫球蛋白M含量递减( P >0.05)。消化酶指标中,胃、肠和肝脏淀粉酶活性随水温骤降温差的增大,均不断下降;胃、肝脏的脂肪酶活性却持续显著增加( P <0.05);胃蛋白酶活性则出现先降后升的趋势( P <0.05)。试验结果表明,急性低温胁迫对子二代中华鲟的抗氧化、免疫和消化酶活性均产生了较大影响,并对其肝脏产生了一定程度的不利影响。因此,在中华鲟的养殖、运输及人工放流过程中,应尽量避免水温骤降造成鱼体损伤。
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| 关 键 词： | 子二代中华鲟 急性低温胁迫 抗氧化指标 免疫指标 消化酶活性 |
Physiological Responses of F2 Chinese Sturgeon Acipenser sinensis to Sharply Low Temperature Stress |
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| GUAN Min,ZHANG Dezhi,WANG Long,BAN Wenbo,ZHANG Houben,GUO Baifu,JIANG Wei.Physiological Responses of F2 Chinese Sturgeon Acipenser sinensis to Sharply Low Temperature Stress[J].Fisheries Science,2019(4):458-464. |
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| Authors: | GUAN Min ZHANG Dezhi WANG Long BAN Wenbo ZHANG Houben GUO Baifu JIANG Wei |
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| Institution: | (Hubei Key Laboratory of Three Gorges Project for Conservation of Fishes,Institute of Chinese Sturgeon,China Three Gorges Corporation,Yichang 443100,China) |
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| Abstract: | In this study,antioxidant indices,immune indices and digestive enzyme activities were investigated in the F2 Chinese sturgeon Acipenser sinensis exposed to sharply low-temperature stress. The F 2 Chinese sturgeon at water temperature of 23 ℃ were directly shifted to a lower temperature water (17 ℃ and 11 ℃) for 2 hours. The results showed that the antioxidant indices of serum and liver were changed significantly in the abrupt decrease temperature groups. As the temperature difference increased,the indices in the serum indicated that superoxide dismutase (SOD) activity was increased first and then decreased. There was significant difference between 11 ℃ group and 17 ℃ group ( P <0.05). The catalase (CAT) activity was changed similarly with SOD activity,and there was significant difference among the groups ( P <0.05). No significant change was observed in the contents of glutathione (GSH) and malondialdehyde (MDA)( P >0.05). Total antioxidant capacity (T-AOC) was increased gradually,significantly lower in 23 ℃ group than other groups ( P <0.05). The SOD activity and CAT activity in the liver was shown to be changed similarly,both increased first and then decreased. The SOD activity was different in 11 ℃ group from other groups significantly ( P <0.05),and the CAT activity in 17 ℃ group was also different from other groups significantly ( P <0.05). The content of GSH was decreased gradually,significantly lower in 23 ℃ group than in other groups ( P <0.05). The content of MDA was increased significantly,significant difference between 23 ℃ group and 17 ℃ group ( P <0.05). The MDA content was different in 11 ℃ group from other groups significantly ( P <0.05). No significant change was observed in T-AOC ( P > 0.05 ). However,as the temperature difference increased,the lysozyme(LZM)activity in serum was initially increased and then decreased significantly ( P <0.05). In the 17 ℃ group,the LZM activity in liver was higher than that in other groups significantly ( P <0.05). No significant change was observed in the content of immunoglobulin M(IgM)( P > 0.05 ). The activity of amylase in the stomach,intestine and liver was reduced significantly with the water temperature difference increased. There was significant difference between 23 ℃ group and 11 ℃ group( P <0.05). The lipase activities in the stomach and liver was increased significantly( P <0.01),and lipase activity was significantly lower in 23 ℃ group than in other groups ( P < 0.05). The gastric protease activity in stomach was initially decreased and then increased significantly( P <0.05). The findings indicated that sharply low temperature stress led to a certain extent adverse effect on the liver,and had a great influence on antioxidant,immune,and the digestive enzyme activities. |
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| Keywords: | Acipenser sinensis sharply low temperature stress antioxidant index immune index digestive enzyme activitiy |
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