| 过瘤胃葡萄糖对围产期奶牛空肠微生物群落和黏膜代谢及免疫相关基因表达的影响 |
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| 引用本文： | 张小丽,吴建,韩雪峰,谭支良,焦金真.过瘤胃葡萄糖对围产期奶牛空肠微生物群落和黏膜代谢及免疫相关基因表达的影响[J].动物营养学报,2019(7):3143-3155. |
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| 作者姓名： | 张小丽 吴建 韩雪峰 谭支良 焦金真 |
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| 作者单位： | 中国科学院亚热带农业生态研究所;中国科学院大学 |
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| 基金项目： | 国家重点研发计划项目(2016YFD0501206);湖南省科技重大专项项目(2017NK1020) |
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| 摘 要： | 本试验旨在探究过瘤胃葡萄糖对围产期奶牛空肠微生物群落和黏膜代谢及免疫相关基因表达的影响。选择10头围产期荷斯坦奶牛4~5岁、体重(515±42)kg]随机分为2组,每组5头。对照组和过瘤胃葡萄糖组奶牛饲喂相同的基础饲粮,同时对照组每头每日给予90 g过瘤胃脂肪,过瘤胃葡萄糖组每头每日给予200 g过瘤胃葡萄糖。预试期14 d(产前第21天到产前第8天),正试期21d(产前第7天到产后第14天)。正式期结束后,将所有奶牛(10头)屠宰并取空肠食糜样品,测定其中挥发性脂肪酸的含量和微生物群落;同时取空肠黏膜样品,进行代谢及免疫相关基因表达分析。结果显示:与对照组相比,添加过瘤胃葡萄糖有降低空肠食糜中总挥发性脂肪酸含量的趋势(P=0.094)。与对照组相比,添加过瘤胃葡萄糖有上调空肠黏膜中葡萄糖转运蛋白4(GLUT4)表达量的趋势(P=0.063),有下调Toll样受体4(TLR4)(P=0.063)、白细胞介素-17A(IL 17A)(P=0.063)和白细胞介素-26(IL 26)(P=0.063)表达量的趋势,同时显著性地上调了丙酮酸羧化酶(PC)(P=0.016)、闭合蛋白(occludin)(P=0.016)和胰岛素生长因子结合蛋白5(IGFBP5)(P=0.032)的表达量,显著性地下调了白细胞介素-22(IL 22)(P=0.016)、基质金属蛋白酶1(MMP1)(P=0.016)和基质金属蛋白酶9(MMP9)(P=0.032)的表达量。此外,与对照组相比,添加过瘤胃葡萄糖显著性地增加了空肠食糜中梭菌属ⅪⅤ子集的数量(P=0.036)。由此可见,围产期奶牛饲粮中添加过瘤胃葡萄糖可以改善葡萄糖在机体的代谢以及提高免疫力,同时促进免疫相关有益菌梭菌属ⅪⅤ子集的定植,从而缓解围产期能量负平衡导致的不良影响。
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| 关 键 词： | 围产期 奶牛 空肠 基因表达 代谢 免疫 微生物 |
Effects of Rumen-Protected Glucose on Jejunal Bacterial Community and Expression of Genes Involved in Mucosal Metabolism and Immune of Dairy Cows in Transition Period |
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| ZHANG Xiaoli,WU Jian,HAN Xuefeng,TAN Zhiliang,JIAO Jinzhen.Effects of Rumen-Protected Glucose on Jejunal Bacterial Community and Expression of Genes Involved in Mucosal Metabolism and Immune of Dairy Cows in Transition Period[J].Acta Zoonutrimenta Sinica,2019(7):3143-3155. |
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| Authors: | ZHANG Xiaoli WU Jian HAN Xuefeng TAN Zhiliang JIAO Jinzhen |
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| Institution: | (Scientific Observing and Experimental Station of Animal Nutrition and Feed Science in South-Central of Ministry of Agriculture, Hunan Provincial Engineering Research Center for Healthy Livestock and Poultry Production,National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production, Key Laboratory of Agro-Ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha 410125, China;University of Chinese Academy of Sciences, Beijing 100049, China) |
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| Abstract: | ZHANG Xiaoli;WU Jian;HAN Xuefeng;TAN Zhiliang;JIAO Jinzhen(Scientific Observing and Experimental Station of Animal Nutrition and Feed Science in South-Central of Ministry of Agriculture, Hunan Provincial Engineering Research Center for Healthy Livestock and Poultry Production,National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production, Key Laboratory of Agro-Ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha 410125, China;University of Chinese Academy of Sciences, Beijing 100049, China) |
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| Keywords: | transition period dairy cows jejunum gene expression metabolism immune microflora |
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