| 中国林蛙真伪品的PCR鉴别 |
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| 引用本文： | 郭立宏,杜智恒,宁方勇,白秀娟.中国林蛙真伪品的PCR鉴别[J].中国畜牧兽医,2010,37(5):96-98. |
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| 作者姓名： | 郭立宏 杜智恒 宁方勇 白秀娟 |
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| 作者单位： | （1.黑龙江省畜牧研究所， 齐齐哈尔 161005； 2.东北农业大学动物科技学院， 哈尔滨 150030） |
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| 摘 要： | 本研究根据GenBank发表的哺乳类SRY核酸序列和禽类EcoRI家族序列设计1对引物,对中国林蛙真伪品进行了PCR扩增,并对特征条带进行了克隆测序。结果表明,PCR扩增产物有明显的区别,中国林蛙、黑龙江林蛙、黑斑蛙和中华大蟾蜍分别扩增出约1000、250、450、750bp的特征条带,通过对中国林蛙1000bp条带的克隆序列进行BLAST比对分析,找到了一些禽类的WZ染色体上的相关基因片段。
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| 关 键 词： | 中国林蛙 伪品 PCR鉴别 |
PCR Identification of True and False of Rana Chensinensis |
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| GUO Li-hong,DU Zhi-heng,NING Fang-yong,BAI Xiu-juan.PCR Identification of True and False of Rana Chensinensis[J].China Animal Husbandry & Veterinary Medicine,2010,37(5):96-98. |
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| Authors: | GUO Li-hong DU Zhi-heng NING Fang-yong BAI Xiu-juan |
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| Institution: | （1．Institute of Animal Science of Heilongjiang Province,Qiqihar 161005,China；2．Department of Animal Science, Northeast Agricultural University, Harbin 150030,China） |
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| Abstract: | In this study, one pairs of primers was designed and synthesized according to the sequence of Mammalia SRY nucleic acid and bird EcoR I published on GenBank, to amplify the true or false of Rana Chensinensis by the PCR technique. As a result, the amplified products of the four species had apparent difference. The four species named Rana Chensinensis, Rana amureansis, Rana nigromaculata and Buffo bufo gargarizans amplified four different characteristic spectrum bands separately 1000,250,450 and 750 bp，and to test the sequence by colon about characteristic spectrum band. To take BLAST analysis about Rana Chensinensis’s characteristic spectrum band(1000 bp), and discovered relative gene fragment on WZ chromosome of some birds. |
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| Keywords: | Rana Chensinensis PCR discrimination |
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