| 牛IL-2重组质粒对口蹄疫病毒G-H环多肽免疫小鼠的佐剂效应 |
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| 引用本文： | 葛兰云,李勐,李爽,刘思莹,徐宗香,张润祥,高明春,马波,王君伟.牛IL-2重组质粒对口蹄疫病毒G-H环多肽免疫小鼠的佐剂效应[J].畜牧兽医学报,2009,40(11). |
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| 作者姓名： | 葛兰云 李勐 李爽 刘思莹 徐宗香 张润祥 高明春 马波 王君伟 |
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| 作者单位： | 东北农业大学动物医学学院,哈尔滨,150030 |
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| 基金项目： | 国家科技支撑计划课题，黑龙江省十一五重大科技项目，国家奶牛技术体系 |
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| 摘 要： | 本试验目的是研究牛IL-2重组质粒对口蹄疫病毒(Foot-and-mouth disease virus,FMDV)G-H环多肽免疫小鼠的佐剂效应.作者构建了含有信号肽序列的牛IL-2真核表达质粒pcDNA3.1-pBoIL-2,并体外鉴定其表达,同时原核表达并纯化了Asial型FMDV G-H环多肽.采用不同组合的pcDNA3.1-pBoIL-2、FMDV G-H环多肽分组免疫小鼠,并设阴、阳性对照,通过间接ELISA试验、微量血清中和试验、淋巴细胞增殖试验和实时定量PCR试验对各免疫组小鼠体液和细胞免疫水平进行综合评价.结果表明,与G-H+pcDNA3.1免疫组比较,G-H+pcD-NA3.1-pBoIL-2免疫组特异性抗体水平、中和抗体滴度、淋巴细胞增殖水平和细胞因子相对表达水平显著提高(P<0.05).结果提示,牛IL-2真核表达质粒可以显著提高G-H环多肽的免疫效果.
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| 关 键 词： | G-H环 多肽 |
Effects of Recombinant Bovine IL-2 on Immune Response of FMDV G-H Loop Peptide in Mice |
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| GE Lan-yun,LI Meng,LI Shuang,LIU Si-ying,XU Zong-xiang,ZHANG Run-xiang,GAO Ming-chun,MA Bo,WANG Jun-wei.Effects of Recombinant Bovine IL-2 on Immune Response of FMDV G-H Loop Peptide in Mice[J].Acta Veterinaria et Zootechnica Sinica,2009,40(11). |
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| Authors: | GE Lan-yun LI Meng LI Shuang LIU Si-ying XU Zong-xiang ZHANG Run-xiang GAO Ming-chun MA Bo WANG Jun-wei |
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| Abstract: | To study the immune response induced by G-H loop peptide of Asial foot-and-mouth disease virus (FMDV) with BOIL-2 recombinant plasmid as adjuvant, the immune effects in im-munized mice were investigated. Recombinant plasmids pcDNA3.1-pBoIL-2 was constructed and the condition of the expression was identified in vitro. G-H peptide was expressed in Rosetta (DE3)pLysS and were successfully purified through GST affinity purification. Five groups of Kunming mice each with 8 were inoculated with different combinations including PBS, pcD-NA3.1-pBoIL-2 and/or G-H peptide, subsequently, immune effects have been evaluated through indirect ELISA assay, the neutralization test, lymphocytes proliferation assay and Quantitive Re-al-time PCR. The results showed that in comparing with the group immunized with G-H+pcD-NA3.1, the group communication with pcDNA3.1-pBoIL-2 induced significantly humoral and cellular immune responses. The results of this study indicate that the co-inoculation of G-H loop peptide with rBoIL-2 (as adjuvant) showed good immune effect. |
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| Keywords: | IL-2 FMDV IL-2 FMDV G-H loop peptide |
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