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Preliminary study on performance of triploid Thai silver barb, Puntius gonionotus |
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| Authors: | Worawut Koedprang Uthairat Na-Nakorn |
| Institution: | a Faculty of Science and Fisheries Technology, Rajamangala Institute of Technology, Trang, Thailand b Department of Aquaculture, Faculty of Fisheries, Kasetsart University, Bangkok 10900, Thailand |
| Abstract: | The triploid chromosome condition was induced in Thai silver barb (Puntius gonionotus) by application of cold shock (2°C) to eggs at time intervals after activation of 0.5 min with a duration of 10 min which resulted in mean triploidy yield of 72.5% at 9 months of age. Growth rate of the 2–9-month-old, cold shock group (0.1–6.2 g/month) did not differ from that of the control (0.1–5.7 g/month). Gonadal somatic indices (GSI) of presumed triploid males and females were lower than that of control (GSI values of the presumed triploids were 35.0–60.2% and 28.7–75.9% of control males and females, respectively). Spermatogenesis and oogenesis were retarded in triploids. However, all stages of spermatogenic cells were observed in triploid males, including few spermatozoa. Oocytes of triploid females did not undergo vitellogenesis while normal oogenesis was observed in diploids. Nuclear volume of red blood cells (RBCs) of triploid fish was 1.63 times larger than that of diploids. |
| Keywords: | Triploid Cold shock Puntius gonionotus |
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