| 丹参3个R2R3-MYB转录因子的亚细胞定位和自激活活性分析 |
| |
| 引用本文： | 丁 恺,麻鹏达,贾彦彦,裴天林,白朕卿,梁宗锁.丹参3个R2R3-MYB转录因子的亚细胞定位和自激活活性分析[J].西北农业学报,2018,27(4):586-594. |
| |
| 作者姓名： | 丁 恺 麻鹏达 贾彦彦 裴天林 白朕卿 梁宗锁 |
| |
| 作者单位： | 西北农林科技大学生命科学学院;浙江理工大学生命科学学院 |
| |
| 基金项目： | 国家自然科学基金（81373908，31670295）；中央高校基本科研业务费专项资金（2452017159）。 |
| |
| 摘 要： | R2R3-MYB转录因子能够有效调控丹参酚酸类物质的积累。将丹参的SmMYB33、SmMYB54和SmMYB93与拟南芥的R2R3-MYB进行比对,找到拟南芥中与丹参3个转录因子亲缘关系最近的功能已知的转录因子,根据这些功能已知的转录因子,对丹参3个转录因子的功能进行预测。构建含有绿色荧光报告基因的载体pA7-GFP-SmMYB33、pA7-GFP-SmMYB54和pA7-GFP-SmMYB93,使用基因枪方法将载体分别转化洋葱表皮细胞进行亚细胞定位分析,结果表明3个转录因子主要定位在细胞核,绿色荧光也有少部分出现在细胞质中。构建酵母表达载体pDEST-GBKT7-SmMYB33、pDEST-GBKT7-SmMYB54和pDESTGBKT7-SmMYB93用于酵母自激活活性分析,结果表明SmMYB33有自激活活性,SmMYB54和SmMYB93没有自激活活性。
|
| 关 键 词： | 丹参 R2R3-MYB转录因子 二级结构分析 亚细胞定位 自激活活性分析 |
Subcellular Localization and Transactivation Analysis of Three R2R3-MYB in Salvia miltiorrhiza Bunge |
| |
| DING Kai,MA Pengd,JIA Yanyan,PEI Tianlin,BAI Zhenqing and LIANG Zongsuo.Subcellular Localization and Transactivation Analysis of Three R2R3-MYB in Salvia miltiorrhiza Bunge[J].Acta Agriculturae Boreali-occidentalis Sinica,2018,27(4):586-594. |
| |
| Authors: | DING Kai MA Pengd JIA Yanyan PEI Tianlin BAI Zhenqing and LIANG Zongsuo |
| |
| Abstract: | R2R3-MYB can regulate accumulation of phenolic acids efficiently in Salvia miltiorrhiza Bunge.In this study,the amino acid sequences of SmMYB33,SmMYB54,SmMYB93 and R2R3-MYBs of Arabidopsis thaliana were aligned to find the R2R3-MYBs of Arabidopsis thaliana which had the closest relationships with the three MYBs of S.miltiorrhiza.According to the phylogenetic analysis,the functions of three MYBs were predicted.The plasmids pA7-GFP-SmMYB36,pA7-GFP-SmMYB54 and pA7-GFP-SmMYB93 were constructed and transiently transformed into onion epidermis to conduct subcellular localization analysis by using gene gun.The results showed that three MYBs were mainly localized in the nucleus of onion epidermis,and a small amount of the GFP fluorescence also appeared in the cytoplasm.The plasmids pDEST-GBKT7-SmMYB36,pDEST-GBKT7-SmMYB54 and pDEST-GBKT7-SmMYB93 were constructed for transactivation analysis.The results implied that SmMYB54 and SmMYB93 did not have the transactivation activity,and SmMYB33 had the transactivation activity. |
| |
| Keywords: | |
| 本文献已被 CNKI 等数据库收录！ |
| 点击此处可从《西北农业学报》浏览原始摘要信息 |
| 点击此处可从《西北农业学报》下载免费的PDF全文 |
|
