| 非放射性ECL直接核酸标记及检测技术在水稻RFLP分析中的应用 |
| |
| 引用本文： | 张少红,朱小源.非放射性ECL直接核酸标记及检测技术在水稻RFLP分析中的应用[J].中国水稻科学,1998,12(1):59-61. |
| |
| 作者姓名： | 张少红 朱小源 |
| |
| 作者单位： | [1]广东省农业科学院水稻研究所广东省水稻细胞工程育种重 [2]广东省农业科学院植物保护研 |
| |
| 摘 要： | 通过用非放射性ECL直接核酸标记及检测技术对水稻品种进行了RFLP分析，介绍了这种简单、安全、快捷的非放射性核酸标记技术。
|
| 关 键 词： | 非放射性 ECL直接核酸标记及检测 限制性片段长度多态性(RFLP) |
| 收稿时间： | 1900-01-01; |
Use of Non-Radioactive ECL Direct Nucleic Acid Labelling and Detection System on RFLP Analysis in Rice |
| |
| ZHANG Shaohong, LIU Bin , LUO Lin , ZHU Xiaoyuan , YANG Qiyun, WU Shangzhong.Use of Non-Radioactive ECL Direct Nucleic Acid Labelling and Detection System on RFLP Analysis in Rice[J].Chinese Journal of Rice Science,1998,12(1):59-61. |
| |
| Authors: | ZHANG Shaohong LIU Bin LUO Lin ZHU Xiaoyuan YANG Qiyun WU Shangzhong |
| |
| Abstract: | Non-radioactive ECL direct nucleic acid labelling and detection system was used for RFLP analysis inrice. The system involves directly labelling DNA or RNA probe with the enzyme horseradish peroxidase (HRP),and the detection of non-radioactive signal is based on chemiluminescence , that is the generation of light via enzyme(HRP)-catalyzed reactions. This is a simple, safe and quick method. The band patterns were clear and comparablewith those by ~32P labelling. |
| |
| Keywords: | Non-radioactive ECL direct nucleic acid labelling and detection RFLP |
| 本文献已被 CNKI 维普 等数据库收录！ |
| 点击此处可从《中国水稻科学》浏览原始摘要信息 |
| 点击此处可从《中国水稻科学》下载免费的PDF全文 |
