副溶血性弧菌分子检测与分型研究进展 |
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引用本文: | 夏楚才,陈水波,苏华,陈丹阳,任明月,叶应旺.副溶血性弧菌分子检测与分型研究进展[J].现代农业科技,2012(22):277-278,280. |
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作者姓名: | 夏楚才 陈水波 苏华 陈丹阳 任明月 叶应旺 |
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作者单位: | 合肥工业大学生物与食品工程学院 |
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基金项目: | 广东省科技计划项目(2011B020314001);合肥工业大学创新实验项目(2011CXCY381) |
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摘 要: | 副溶血性弧菌是一种革兰氏阴性嗜盐菌,常通过食用被该菌污染的食品导致食物中毒。该文综述了副溶血性弧菌的分子检测技术如PCR、环介导等温扩增、免疫捕获等,及分型技术如RAPD-PCR、ERIC-PCR、PFGE、核糖体分型、RFLP等,这些方法将为副溶血性弧菌食物中毒提供重要的检测与分型手段,对预防与控制副溶血性弧菌食物中毒具有重要意义。
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关 键 词: | 副溶血性弧菌 检测 分型方法 |
Research Progress of Detection and Typing of Vibrio parahaemolyticus |
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Authors: | XIA Chu-cai CHEN Shui-bo SU Hua CHEN Dan-yang REN Ming-yue YE Ying-wang |
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Institution: | (School of Biotechnology and Food Engineering,Hefei University of Technology,Hefei Anhui 230009) |
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Abstract: | Vibrio parahaemolyticus(VP) is a halophilic bacterium.Food poisoning frequently caused by food contaminated by vibrio parahaemol-yticus.The detection and typing approaches were reviewed in this paper including PCR,LAMP,RAPD-PCR,ERIC-PCR,PFGE,RFLP and Ribotyping.These methods played an important role in preventing and controlling of food-poisoning outbreaks caused by VP. |
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Keywords: | Vibrio parahaemolyticus detection typing approaches |
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