The effect of ovule age on ovary-slice culture and ovule culture in intraspecific and interspecific crosses with Tulipa gesneriana L. |
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| Authors: | MGM Van Creij DMFJ Kerckhoffs JM Van Tuyl |
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| Institution: | (1) Centre for Plant Breeding and Reproduction Research (CPRO-DLO), P.O. Box 16, 6700 AA Wageningen, The Netherlands |
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| Abstract: | The efficiency of two embryo rescue techniques, direct ovule culture and ovary-slice culture followed by ovule culture, is
studied in crosses with Tulipa gesneriana as maternal genotype. The germination percentages increased, in most cases, significantly with increasing ages of te ovary-slices
and ovules at the start of the cultures. The low number of embryos recovered at early culture dates is caused by a higher
rate of embryo abortion and by retarded embryo development. The germination percentages for ovary-slice culture followed by
ovule culture was mostly comparable to direct ovule culture. Unique hybrids have been obtained from the crosses T. gesneriana × T. agenensis and T. gesneriana × T. praestans.
This revised version was published online in July 2006 with corrections to the Cover Date. |
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| Keywords: | embryo development embryo rescue hybridization tulip |
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