Shoot production from onion callus tissue cultures |
| |
| Authors: | DI Dunstan KC Short |
| |
| Institution: | Department of Biology, Division of Plant Science, North East London Polylechnic, Romford Road, Stratford, London E15 4LZ Gt. Britain |
| |
| Abstract: | Shoots have been produced on callus derived from onion set and from seedling radicle tissue. Whilst callus of set origin responded optimally to medium containing the cytokinin 6-(3-methyl-2-buten-1-ylamino)-purine (2iP) at 2.00 mg l?1 and naphthaleneacetic acid (NAA) at 0.06 mg l?1, seedling radicle callus showed a range of response. The medium used for callus initiation, the age of callus, and the provision of a dark period following inoculation on to the organogenesis medium have been shown to be critical for shoot formation. A limited number of embryoids have been produced from cultures; their occurrence was usually associated with an increase in shoot numbers for the generative callus tissue. Meristemoid areas have been observed in light micrographs of callus cultured upon organogenesis media. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
