| 重组PEA受体结合区蛋白的免疫活性 |
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| 引用本文： | 万忠海,金爱军,谭建华,江禹,刘晓明,朱平.重组PEA受体结合区蛋白的免疫活性[J].中国兽医学报,2004,24(1):82-84. |
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| 作者姓名： | 万忠海 金爱军 谭建华 江禹 刘晓明 朱平 |
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| 作者单位： | 1. 解放军军需大学,军事兽医研究所,吉林,长春,130062
2. 长春职工医科大学,吉林,长春,130051 |
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| 基金项目： | 国家自然科学基金资助项目 ( 3 9770 866) |
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| 摘 要： | 为了观察重组绿脓杆菌外毒素 A(PEA)受体结合区蛋白在不同动物体内的免疫效果 ,将该蛋白纯化后免疫了小鼠、家兔、山羊等动物。对小鼠进行了攻毒实验 ,通过 EL ISA方法检测了家兔、山羊血清中抗体的消长规律。结果对小鼠的保护情况为 :最后 1次免疫后 14 d小鼠对 3倍 L D50 的 PEA攻毒保护率为 10 0 % ,2 1d保护率为 5 0 % ,2 8d后无保护效果。家兔和山羊免疫后 7～ 14 d达到峰值 ,2 1d后逐渐降低 ,2 8～ 35 d后降至免疫前水平
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| 关 键 词： | 重组绿脓杆菌外毒素A 受体结合区蛋白 免疫活性 |
| 文章编号： | 1005-4545(2004)01-0082-02 |
| 修稿时间： | 2003年3月28日 |
The Immunocompetent Experiment of the Recombinant Receptor Binding Protein of the Exotoxin A of P.aeruginosa |
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| WAN Zhong-hai,JIN Ai-jun,TAN Jian-hua,JIANG Yu,LIU Xiao-ming,ZHU Ping.The Immunocompetent Experiment of the Recombinant Receptor Binding Protein of the Exotoxin A of P.aeruginosa[J].Chinese Journal of Veterinary Science,2004,24(1):82-84. |
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| Authors: | WAN Zhong-hai JIN Ai-jun TAN Jian-hua JIANG Yu LIU Xiao-ming ZHU Ping |
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| Institution: | WAN Zhong-hai~1,JIN Ai-jun~2,TAN Jian-hua~1,JIANG Yu~1,LIU Xiao-ming~1,ZHU Ping~1 |
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| Abstract: | For observing the immune result of the recombinant receptor binding protein of the exotoxin A of P.aeruginosa in different animals,the mice,rabbits and goats were immunized with the purified protein,then challenged with PEA.The anti-PEA antibody level in the rabbits and goats was analyzed by indirect ELISA.The protection rate in the mice was 100% at 14th day,50% at 21th day and no protection after 28 days;the highest antibody level in the rabbits and goats was observed during 7-14 days postinoculation,and then dropped gradually from 21 days after the last inoculation. |
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| Keywords: | PEA receptor binding protein immunocompetence |
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