| 微波诱变选育高效溶磷木霉菌株的研究 |
| |
| 引用本文： | 薛应钰,叶巍,张树武,刘佳,徐秉良.微波诱变选育高效溶磷木霉菌株的研究[J].干旱地区农业研究,2016,34(4):231-236. |
| |
| 作者姓名： | 薛应钰 叶巍 张树武 刘佳 徐秉良 |
| |
| 作者单位： | 甘肃农业大学植物保护学院,甘肃 兰州,730070 |
| |
| 基金项目： | 甘肃省高校基本科研业务费项目资助(甘财教[2012]129号)；甘肃省教育厅高校科研项目资助(2013A －061)；甘肃省自然科学基金项目(145 RJZA095)；甘肃农业大学科技创新基金资助项目(GAU－CX1014) |
| |
| 摘 要： | 为了获得高效溶磷菌株,对木霉菌T6菌株进行微波诱变处理,测定并分析了微波输出功率和辐照时间等参数对诱变效果的影响。结果表明:最佳诱变条件是微波输出功率900 W,辐照时间70 s,且在此条件下得到8株具有较高溶磷能力的突变菌株T6-9、T6-33、T6-93、T6-126、T6-157、T6-188、T6-196和T6-203。经过多代转接培养最后得到一株遗传稳定的溶磷木霉菌株T6-157,该菌株在液体培养条件下溶磷量为204.46 mg·L~(-1),与出发菌株相比,溶磷量提高了107.97%,植酸酶活性提高了57.35%,且突变菌株对土传植物病原菌的拮抗能力也有所提高。
|
| 关 键 词： | 木霉菌 微波诱变 溶磷能力 拮抗作用 |
Screening of Trichoderma sp. strain for high phosphorus solubilization using microwave mutagenesis |
| |
| XUE Ying-yu,YE Wei,ZHANG Shu-wu,LIU Ji,XU Bing-liang.Screening of Trichoderma sp. strain for high phosphorus solubilization using microwave mutagenesis[J].Agricultural Research in the Arid Areas,2016,34(4):231-236. |
| |
| Authors: | XUE Ying-yu YE Wei ZHANG Shu-wu LIU Ji XU Bing-liang |
| |
| Institution: | College of Plant Protection, Gansu Agricultural University, Lanzhou, Gansu 730030, China,College of Plant Protection, Gansu Agricultural University, Lanzhou, Gansu 730030, China,College of Plant Protection, Gansu Agricultural University, Lanzhou, Gansu 730030, China,College of Plant Protection, Gansu Agricultural University, Lanzhou, Gansu 730030, China and College of Plant Protection, Gansu Agricultural University, Lanzhou, Gansu 730030, China |
| |
| Abstract: | In order to obtain Trichoderma sp .strain with high phosphorus dissolving efficiency ,the original strain , Trichoderma T6 with low ability in dissolving phosphorus ,was mutated by microwave .The microwave mutagenesis param-eters were optimized ,and the genetic stability ,phosphate solubilizing power and antibiotic ability of the mutant were in-vestigated .The results showed that the optimum mutagenic method was with an irradiation power at 900 W and a mutation time of 70 s .8 stains with high phosphorus solubilizing capability named T 6-9 ,T6-33 ,T6-93 ,T6-126 ,T6-157 ,T6-188 ,T6-196 and T6-203 were obtained using the optimized mutagenic method .After several generations , a strain named T6-157 with relatively stable hereditary was eventually obtained ,exhibiting high phosphorus solubilizing capability .The highest phosphate solubilizing power of mutant strain T 6-157 was 204 .46 mg·L-1 ,and the phosphate solubilizing power and phytase activity were increased by 107 .97% and 57 .35% ,respectively ,compared with the origi-nal strain .The antagonistic ability against the main soil-borne pathogens was higher than that of the initial strain T 6 . |
| |
| Keywords: | Trichoderma sp microwave mutagenesis phosphate solubilization capacity antagonistic effect |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
| 点击此处可从《干旱地区农业研究》浏览原始摘要信息 |
| 点击此处可从《干旱地区农业研究》下载免费的PDF全文 |
|
