| 水分胁迫下小麦TaWSI18基因的电子克隆及生物信息学分析 |
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| 引用本文： | 樊磊,陈娟,张艳娥,张林生,朱维宁,张大鹏.水分胁迫下小麦TaWSI18基因的电子克隆及生物信息学分析[J].干旱地区农业研究,2016,34(4):69-76. |
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| 作者姓名： | 樊磊 陈娟 张艳娥 张林生 朱维宁 张大鹏 |
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| 作者单位： | 西北农林科技大学生命科学学院 旱区作物逆境生物学国家重点实验室,陕西 杨凌,712100 |
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| 基金项目： | 国家自然科学基金资助项目(31071349)；高等学校博士学科点专项基金(20120204110033) |
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| 摘 要： | 利用电子克隆和RT-PCR技术,从干旱胁迫的小麦叶片中分离出4条新的具有高度同源性的WSI18基因,即TaWSI18-1、TaWSI18-2、TaWSI18-3和TaWSI18-4(Gene Bank登陆号分别为:KP226849、KP226850、KP226851和KP226852);其开放阅读框均为678 bp,编码225个氨基酸。生物信息学分析表明,该类蛋白具有亲水性,存在多个磷酸化位点,无信号肽结构域及剪切位点,定位于细胞质中,无跨膜结构域;二级结构分析表明,该蛋白α螺旋和无规则卷曲的比例达到90%以上;同源性比较及聚类分析表明,小麦TaWSI18蛋白同无芒雀麦(Bromus inermis,BAN15016)WSI18蛋白和二穗短柄草(Brachypodium distachyon,XP 003569641)LEA3蛋白具有高度的同源性,相似性分别为94%和70%。小麦TaWSI18基因编码蛋白的氨基酸序列的N端存在与LEA蛋白的N端区域高度保守的序列。表明小麦TaWSI18蛋白的性质与LEA3蛋白的相似,说明了小麦的TaWSI18蛋白可能与LEA3蛋白存在相同或相似的功能,为进一步研究其在小麦水分胁迫下的功能奠定基础。
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| 关 键 词： | 小麦 水分胁迫 TaWSI18基因 电子克隆 生物信息学 |
Identification and characterization of water stress-induced TaWSI18 gene in silico cloning from Triticum aestivum and its bioinformatic analysis |
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| FAN Lei,CHEN Juan,ZHANG Yan-e,ZHANG Lin-sheng,ZHU Wei-ning,ZHANG Da-peng.Identification and characterization of water stress-induced TaWSI18 gene in silico cloning from Triticum aestivum and its bioinformatic analysis[J].Agricultural Research in the Arid Areas,2016,34(4):69-76. |
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| Authors: | FAN Lei CHEN Juan ZHANG Yan-e ZHANG Lin-sheng ZHU Wei-ning ZHANG Da-peng |
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| Institution: | State Key Laboratory of Crop Stress Biology in Arid Area, College of Life Sciences, Northwest A & F University, Yangling, Shaanxi 712100, China,State Key Laboratory of Crop Stress Biology in Arid Area, College of Life Sciences, Northwest A & F University, Yangling, Shaanxi 712100, China,State Key Laboratory of Crop Stress Biology in Arid Area, College of Life Sciences, Northwest A & F University, Yangling, Shaanxi 712100, China,State Key Laboratory of Crop Stress Biology in Arid Area, College of Life Sciences, Northwest A & F University, Yangling, Shaanxi 712100, China,State Key Laboratory of Crop Stress Biology in Arid Area, College of Life Sciences, Northwest A & F University, Yangling, Shaanxi 712100, China and State Key Laboratory of Crop Stress Biology in Arid Area, College of Life Sciences, Northwest A & F University, Yangling, Shaanxi 712100, China |
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| Abstract: | WSI18 ,a water stress -induced genes ,plays an important role in plants under water deficit .Silico cloning and RT-PCR were taken to isolate four new highly homologous WSI18 genes:TaWSI18- 1 , TaWSI18- 2 , TaWSI18- 3 and TaWSI18 - 4 (GenBank accession number :KP226849 ,KP226850 ,KP226851and KP226852 ) in wheat ,ORFs of which are all 678 bp encoding 225 amino acids .Bioinformatic analysis showed that they are highly hy-drophilic and have multiple phosphorylation sites .They are located to cytoplasm and have no transmembrane domain sig-nal peptides ,as well as no splicing sites .The proportion of αhelix and random coil are over 90% by a secondary struc-ture prediction .Homology comparison and phylogenetic analysis showed that TaWSI 18 proteins are highly homologous to WSI18 of Bromus inermis and LEA3 Brachypodium distachyon ,sharing 94% and 70% similarities respectively .In addi-tion ,TaWSI18 proteins have conserved amino acid sequences common to LEA protein in the N-terminal region .They also have a similar character to LEA3 protein by bioinformatic analysis that TaWSI18 proteins may have the same or similar function to LEA3 protein ,which lies a foundation for the future study on its function under water-stress . |
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| Keywords: | Triticum aestivum water stress TaWSI18 silico cloning bioinformatics |
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