| 载铜蒙脱石对嗜水气单胞菌粘附尼罗罗非鱼上皮细胞的影响 |
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| 引用本文： | 胡彩虹.载铜蒙脱石对嗜水气单胞菌粘附尼罗罗非鱼上皮细胞的影响[J].水产学报,2005,29(5):619-623. |
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| 作者姓名： | 胡彩虹 |
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| 作者单位： | 浙江大学动物科学学院动物分子营养学教育部重点实验室,浙江,杭州,310029;浙江大学动物科学学院动物分子营养学教育部重点实验室,浙江,杭州,310029;浙江大学动物科学学院动物分子营养学教育部重点实验室,浙江,杭州,310029;浙江大学动物科学学院动物分子营养学教育部重点实验室,浙江,杭州,310029 |
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| 基金项目： | 国家自然科学基金项目（30471255）;浙江省科技厅重点攻关项目（2005C22054） |
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| 摘 要： | 采用尼罗罗非鱼上皮细胞培养模型，观察嗜水气单胞菌对鳃上皮、皮肤上皮、肠上皮细胞的粘附率，研究载铜蒙脱石对细菌粘附的阻断作用及其对细菌粘附引起鱼上皮细胞膜生物特性变化的影响。结果显示：嗜水气单胞菌与鱼上皮细胞均有不同程度的粘附作用，粘附率大小顺序为鳃上皮〉皮肤上皮〉肠上皮细胞。载铜蒙脱石均显著降低了嗜水气单胞菌对鳃、皮肤和肠上皮细胞的粘附率（P〈0．05），而且对不同上皮细胞的粘附阻断率无显著差异。嗜水气单胞菌粘附肠上皮细胞后细胞膜生物特性发生了显著变化，与正常对照组相比，嗜水气单胞菌粘附鱼上皮细胞后，胞浆游离钙和细胞膜磷脂酶A2活性显著上升。载铜蒙脱石可显著降低由于嗜水气单胞菌粘附鱼上皮细胞引起的胞浆游离钙和细胞膜磷脂酶A2活性的升高。结果表明，载铜蒙脱石可有效阻断病原菌粘附，从而防治细菌感染和细菌移位。
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| 关 键 词： | 载铜蒙脱石 嗜水气单胞菌 粘附 鱼上皮细胞 细胞膜 |
| 文章编号： | 1000-0615（2005）05-0619-05 |
| 收稿时间： | 2004-09-09 |
| 修稿时间： | 2004年9月9日 |
Effects of Cu bearing montmorillonite on Aeromonas hydrophila adhesion to epithelial cells of Nile tilapia |
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| HU Cai-hong.Effects of Cu bearing montmorillonite on Aeromonas hydrophila adhesion to epithelial cells of Nile tilapia[J].Journal of Fisheries of China,2005,29(5):619-623. |
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| Authors: | HU Cai-hong |
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| Institution: | Key Laboratory of Molecular Animal Nutrition of Ministry of Education, College of Animal Science, ZheJiang University, Hangzhou 310029, China |
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| Abstract: | Skin,gill and intestinal epithelial cells were developed using primary cultures of mucosal cells(isolated from healthy Nile tilapia) to study the adhesion of Aeromonas hydrophila to fish epithelial cells and the effects of Cu bearing montmorillonite(Cu-MMT) on the adhesion of bacteria and on cell membrane injury due to Aeromonas hydrophila.The three primary-culture cells are susceptible to Aeromonas hydrophila attachment,with the greatest binding affinity found in gills,and to a lesser extent,in skin and intestine epithelial cells.Cu-MMT significantly inhibited(P<0.05) adhesion of bacteria to skin,gill and intestinal epithelial cells.The cell membrane was injured due to Aeromonas hydrophila.The activity of cytosolic free calcium concentration and membranous phospholipase A_2 were increased(P<0.05) significantly.Cu-MMT can significantly reduced(P<0.05) cell membrane injury caused by the adhesion of Aeromonas hydrophila.Our study shows that Cu-MMT showed a strong ability to inhibition adhesion of pathogenic strains to fish epithelial cell and open the possibility of applying Cu-MMT in aquaculture. |
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| Keywords: | Cu bearing montmorillonite Aeromonas hydrophila adhesion fish epithelial cells cell membrane |
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