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贵阳地区新发生猪传染病的血清学检测
引用本文:岳筠,周碧君,徐春志,史开志.贵阳地区新发生猪传染病的血清学检测[J].贵州大学学报(农业与生物科学版),2007,26(3):215-219.
作者姓名:岳筠  周碧君  徐春志  史开志
作者单位:1. 贵州大学,动物科学学院,贵州,贵阳,550025
2. 贵州大学,动物疫病研究所,贵州,贵阳,550025
基金项目:贵州省农业科技攻关重大项目
摘    要:采用血清学方法对贵阳地区部分种猪场、规模饲养场及农村散养户猪群分别进行PPV、PRV、PRRS、PCV及APP抗体水平监测。以乳胶凝集试验检测PPV抗体阳性率为0%(0/160);以间接ELISA检测PCV和PRRS的抗体阳性率分别为14%(28/200)和0.62%(1/160);以竞争ELISA检测PRV的gpI抗体阳性率为19.14%(36/188);以间接血凝试验检测APP的抗体阳性率为19.14%(36/188)。结果表明,PPV和PRRS似乎不是引起贵阳地区猪繁殖障碍的主要原因,而PRV和APP在非免疫猪群中的抗体阳性率较高,应引起该地区养猪业的高度重视。

关 键 词:  传染病  血清学  抗体水平  贵阳
文章编号:1008-0457(2007)03-0215-05
收稿时间:2007-03-13
修稿时间:2007-03-132007-03-20

The serological detection of newly occurring pig infectious diseases in Guiyang
YUE Jun,ZHOU Bi-jun,XU Chun-zhi,SHI Kai-zhi.The serological detection of newly occurring pig infectious diseases in Guiyang[J].Journal of Mountain Agriculture & Biology,2007,26(3):215-219.
Authors:YUE Jun  ZHOU Bi-jun  XU Chun-zhi  SHI Kai-zhi
Institution:1. College of Animal Science , Guizhou University , Guizhou Guiyang 550025, China; 2. Institute of Animal Disease, Guizhou University, Guizhou Guiyang 550025, China
Abstract:The antibody levels against PPV,PRV,PRRS,PCV and APP were surveyed by serological methods in partial pig breeding farms, large scale farms and countryside individual farmers of Guiyang district. No PPV antibody was observed in pig herds by latex agglutination test. The positive rates of PCV and PRRS were 14% and 0. 62% respectively by indirect ELISA. The gpI antibody against PRV has been detected by competitive ELISA, accounting for 19. 14%. Meanwhile the positive rate of APP antibody also reached 19. 14% by indirect hemoagglutination assay. It seemed that the PPV and PRRS were not the main reasons for pig reproductive disturbanee,more attention should be paid to PRV and APP,because the antibody levels were higher in non-vaccinated pig herds of Guiyang district.
Keywords:pig  infectious disease  serology  antibody level  Guiyang
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