Efficient method of micropropagation and in vitro rooting of teak (Tectona grandis L.) focusing on large-scale industrial plantations |
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| Authors: | Emilio Mendoza de Gyves Juwartina Ida Royani Eddo Rugini |
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| Institution: | 1. Dipartimento Produzione Vegetale, Università degli Studi della Tuscia, Via S. C. de Lellis, 1, 01100, Viterbo, Italy
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| Abstract: | Multiple shoots of high quality were produced in vitro from nodal expiants of Tectona grandis. An average of about 4 shoots/uninodal expiant was obtained within 4 weeks of culture on Murashige and Skoog’s (mMS) medium modified by 50% reduction in NH4NO3 concentration, supplemented with benzylaminopurine (1.5 mg L?1); indole-3-butyric acid (0.01 mg L?1) and gibberellic acid (0.1 mg L?1). The latter was applied both in the medium and by soaking the nodal segments for 10 s. in a gibberellic acid solution of 100 mg L?1. Hundred percent of shoots rooted cultured on modified MS medium containing IBA (0.5 mg L?1) and putrescine (160 mg L?1). Putrescine promoted both strong and highly ramified roots and fast growing shoots during the rooting phase, conditioning the plantlets for a good survival and quality. Plantlets were transferred to jiffy pots for a short acclimatization stage in greenhouse where they survived at 100%. This highly reproducible procedure can be adopted for large scale teak propagation. |
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