| 变异连翘的组织培养及快速繁殖的研究 |
| |
| 引用本文： | 武翼,刘晓霞,冯家祺,徐娜,姜长阳.变异连翘的组织培养及快速繁殖的研究[J].山东林业科技,2009,39(3):41-43,122. |
| |
| 作者姓名： | 武翼 刘晓霞 冯家祺 徐娜 姜长阳 |
| |
| 作者单位： | 辽宁师范大学生命科学学院,辽宁,大连,116029 |
| |
| 摘 要： | 以具有二次开花变异性状的连翘休眠芽为外植体，在附加不同浓度的BA、IAA、IBA、NAA和2，4-D的MS、1／2MS、B5、N6、White培养基上进行离体培养。结果表明：1／2MS＋6-BA0．2mg·L^-1＋NAA0．1mg·L^-1＋IAA0·2mg·L^-1是休眠芽生长的理想培养基；MS＋AgNO30．5mg·L^-1＋6BA1．0mg·L^-1＋NAA0．1mg·L^-1-0．3mg·L^-1是生长芽分化培养和不定芽继代培养的理想培养基；1／2MS＋IAA0．2mg·L^-1NAA0．4mg·L^-1是生根培养和生根继代培养的理想培养基；移植的试管苗保持了二次开花的变异性状。
|
| 关 键 词： | 变异连翘 无性系 快速繁殖 |
The study of Tissue culture and rapid propagation of Forsythia suspensa |
| |
| Wu Yi,Liu Xiaoxia,Feng Jiaqi,Xu Na,Jiang Changyang.The study of Tissue culture and rapid propagation of Forsythia suspensa[J].Journal of Shandong Forestry Science and Technology,2009,39(3):41-43,122. |
| |
| Authors: | Wu Yi Liu Xiaoxia Feng Jiaqi Xu Na Jiang Changyang |
| |
| Institution: | (College of life Science, Liaoning Normal University , Dalian 116029 china) |
| |
| Abstract: | The explants from Forsythia suspensa with variant characters of second flowering were cultured in MS medium supplemented with BA,IAA,NAA and 2,4--D at different levels. The results show that the optimal medium for differentiation of dormant bud is 1/2MS＋6-BA0.2 mg·L^-1 ＋NAA 0. 1mg·L^-1 ＋IAA 0.2mg·L^-1 ;And the optimal medium for differentialation of adventitious bud is MS＋AgNO30.5 mg·L^-1＋6-BA1.0 Mg·L^-1＋NAA0. 1 mg·L^-1-0.3mg·L^-1.The suitable medium for rooting is 1/2MS＋AA0.2 mg·L^-1＋NAA0.4 mg·L^-1. Induction of root is better in medium of 1/ 2MS＋IAA0.2mg/L＋G15g· L^-1. Ashes from a stove were a good transplanting medium. The tuber seedling of open fieid cul tivation keep the variant character. |
| |
| Keywords: | Forsythia suspensa Clone Rapid propagation |
| 本文献已被 维普 万方数据 等数据库收录！ |
|
