| 3α-HSD单克隆抗体的制备及其鉴定 |
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| 引用本文： | 杨伟克,吴兴海,王建华.3α-HSD单克隆抗体的制备及其鉴定[J].天津农业科学,2014(5):31-35. |
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| 作者姓名： | 杨伟克 吴兴海 王建华 |
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| 作者单位： | 山东出入境检验检疫局检验检疫技术中心,山东青岛266002 |
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| 基金项目： | 质检公益性行业科研专项（201110018-04）;质检总局专项（2013IK173、2013IK293） |
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| 摘 要： | 为制备抗3α-HSD蛋白单克隆抗体(MAb),以已表达纯化的高纯度3α-HSD蛋白作为免疫原免疫BALB/c小鼠,利用杂交瘤技术,经过免疫和细胞融合后筛选出特异性的抗3α-HSD蛋白单克隆抗体,成功冻存35株IgG类型阳性杂交瘤细胞株。对14号和20号Anti-3α-HSD进行亲和性测试,发现其与可溶性抗原蛋白的亲和常数分别为3.7E+09和4.2E+09,二者可与抗原牢固结合,具有良好的亲和性。
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| 关 键 词： | 3α-HSD 单克隆抗体 制备 鉴定 |
Development and Identification of Monoclonal Antibody Against 3α-HSD |
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| YANG Wei-ke,WU Xing-hai,WANG Jian-hua.Development and Identification of Monoclonal Antibody Against 3α-HSD[J].Tianjin Agricultural Sciences,2014(5):31-35. |
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| Authors: | YANG Wei-ke WU Xing-hai WANG Jian-hua |
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| Institution: | (Inspection and Quarantine Center,Shandong Exit and Entry Inspection and Quarantine Bureau, Qingdao, Shandong 266002,China) |
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| Abstract: | For development of monoclonal antibody（MAbs） against 3α-HSD, BALB/c mice were immunized with a synthesized 3α- HSD. The splenocytes of the immunized mice were fused with sp2/0 cells,and the positive hybridoma cells were screened. 35 of MAbs were identified as IgG and successfully cryopresevated, the affinity constant of 14# and 20# MAbs against 3α-HSD were 3.7E＋09 and 4.2E＋09.The affinity of MAbs was good. |
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| Keywords: | 3α-HSD monoclonal antibody （MAbs） development identification |
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