| 不同种质荔枝基因组DNA提取研究 |
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| 引用本文： | 曹辉庆,彭宏祥,廖惠红.不同种质荔枝基因组DNA提取研究[J].广西农业科学,2009,40(6):618-620. |
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| 作者姓名： | 曹辉庆 彭宏祥 廖惠红 |
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| 作者单位： | 1. 广西作物遗传改良生物技术重点开放实验室,南宁,530007;广西农科院园艺研究所,南宁,530007
2. 广西农科院园艺研究所,南宁,530007 |
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| 基金项目： | 广西自然科学基金项目 |
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| 摘 要： | 针对荔枝体细胞富含多酚、多糖、色素以及蛋白质等次生物质的特点，以荔枝叶片为材料，分别采用CTAB法和SDS法对荔枝野生、半野生和栽培种质的基因组DNA进行提取。结果表明，SDS法提取的荔枝基因组DNA效果稳定，获得DNA的机率为100％，DNA纯度高、质量好，可用于AFLP酶切、连接、预扩增和选择性扩增，并获得清晰、稳定的扩增产物；CTAB法提取的荔枝基因组DNA效果不稳定，DNA浓度较低，易出现多糖等次生物质与DNA共沉淀现象，无法检测出DNA。因此，SDS法可用于不同荔枝种质基因组DNA的提取，进行AFTJP、SSR、ISSR分析。
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| 关 键 词： | 荔枝 SDS法 CTAB法 DNA提取 AFLP |
Extraction of genomic DNA in different litchi germplasm |
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| CAO Hui-qing,PENG Hong-xiang,LIAO Hui-hong.Extraction of genomic DNA in different litchi germplasm[J].Guangxi Agricultural Sciences,2009,40(6):618-620. |
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| Authors: | CAO Hui-qing PENG Hong-xiang LIAO Hui-hong |
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| Institution: | 1 Guangxi Crop Genetic Improvement and Biotechnology Lab;Nanning 530007;China;2 Horticulture Research Institute;Guangxi Academy of Agricultural Sciences;China |
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| Abstract: | In accordance with large amounts of secondary metabolites such as polyphenol,polysaccharide,pigment and protein,and so on in leaf cells of litchi,the genomic DNA of wild,semi-wild and cultivated verieties of litchi were extracted by SDS and CTAB methods. The results showed that the effects of SDS extraction on genomic DNA of litchi were stable,the genomic DNA with high-quality and high-purity could be obtained and used for AFLP analysis,and the amplified products were clear and stable. The effects of CTAB e... |
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| Keywords: | AFLP |
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