| 呼吸型鸡传染性支气管炎病毒的抗原定位与动态分布 |
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| 引用本文： | 祝玉卿,曾祥伟,张芳,魏萍.呼吸型鸡传染性支气管炎病毒的抗原定位与动态分布[J].现代畜牧兽医,2005(4):6-8. |
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| 作者姓名： | 祝玉卿 曾祥伟 张芳 魏萍 |
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| 作者单位： | 1. 东北农业大学动物医学院,黑龙江,哈尔滨,150030
2. 东北农业大学动物医学院,黑龙江,哈尔滨,150030;东北林业大学野生动物资源学院,黑龙江,哈尔滨,150040 |
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| 基金项目： | 黑龙江省教育厅科学技术研究项目SARS专项资金(2003fz012) |
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| 摘 要： | 应用间接免疫荧光抗体(IFA)和RT-PCR法对人工感染IBV-M41株的SPF鸡不同脏器中的病毒进行了跟踪检测。结果在感染后24h气管中最早出现特异性荧光，感染后第3h肺脏和肾脏出现特异性荧光，感染后第5d肝脏、脾脏、法氏囊、直肠等脏器也不同程度的出现特异性荧光，特异性荧光在气管中最强，持续时间也最长，可达14d或更长：在肺脏和肾脏可持续3～5d；而在其他器官持续1～3d。试验结果表明气管、肺脏、肾脏都是M41株病毒增殖的场所，但随感染时间的延长，病毒最终定位于主要的靶器官气管。IFA和RT-PCR方法均可以对IBV抗原定位，相对来说IFA法更直观，而RT-PCR更灵敏。
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| 关 键 词： | 鸡传染性支气管炎病毒 抗原定位 动态分布 呼吸型 RT-PCR方法 RT-PCR法 特异性 跟踪检测 SPF鸡 人工感染 荧光抗体 间接免疫 持续时间 试验结果 病毒增殖 感染时间 肾脏 肺脏 法氏囊 可持续 M41 靶器官 IBV IFA 脏器 肝脏 |
| 文章编号： | 1672-9692(2005)04-0006-03 |
| 修稿时间： | 2005年1月4日 |
Antigen localization and dynamic distribution of respiratory infectious Bronchitis virus |
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| Zhu Yuqing,Zeng Xiangwei,Zhang Fang,Wei Ping.Antigen localization and dynamic distribution of respiratory infectious Bronchitis virus[J].Modern JOurnal of Animal Husbandry and Veterinary Medicine,2005(4):6-8. |
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| Authors: | Zhu Yuqing Zeng Xiangwei Zhang Fang Wei Ping |
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| Institution: | Zhu Yuqing1,Zeng Xiangwei1,2,Zhang Fang1,Wei Ping1 |
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| Abstract: | IFA and RT-PCR were used to detect difference organs of SPF chickens infected IBV-M41 strain. The results showed that the first bright specific fluorescence (BSF) was observed in chicken trachea epithelial cells with specific staining 24hrs after artificial IBV-infection. The BSF was observed in chicken lungs' and kidneys' cells 3days after artificial IBV-infection. There were different degrees of BSF in cells of liver, spleen, bursa and rectum 5days after artificial IBV-infection. The BSF was most noticeable in trachea and its lasting time could last for 14days or longer. The BSF could last for 3-5days in lung and kidney, and could last for 1-3days in other organs. It was showed that trachea, lungs and kidneys were the place of virus copying, with the time of IBV-infection, the virus were located in the target organ-trachea. IFA and RT-PCR were both used for antigen localization, IFA was more direct, and RT-PCR was more sensitive and specific. |
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| Keywords: | Infectious Bronchitis Virus IFA PCR antigen localization |
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