| 高效液相色谱法测定饲料中阿那曲唑 |
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| 引用本文： | 梁萌,任爱丽,陈淑沂.高效液相色谱法测定饲料中阿那曲唑[J].中国农业科技导报,2008,10(Z2):53-56. |
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| 作者姓名： | 梁萌 任爱丽 陈淑沂 |
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| 作者单位： | 农业部饲料质量监督检验测试中心（济南） |
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| 摘 要： | 称取适量饲料样品,用乙醚和二氯甲烷混合提取液提取后离心,取上层有机相过混合型阳离子交换小柱进行净化处理后,上高效液相色谱仪分析,用紫外检测器和二极管阵列检测器分别在220 nm处进行定量和定性。结果表明,阿那曲唑浓度在0.1~1 000μg/mL范围与峰面积线性良好,回归系数r=0.998 9。回收率为90%~112%,变异系数<5%。阿那曲唑的最低检测限为1μg/mL。4种常用药物在该条件下对阿那曲唑的测定均无干扰。
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| 关 键 词： | 饲料 阿那曲唑 高效液相色谱 |
Determination of Anastrozole in Compound Feed Using HPLC |
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| LIANG Meng,REN Ai-li,Chen Shu-yi.Determination of Anastrozole in Compound Feed Using HPLC[J].Journal of Agricultural Science and Technology,2008,10(Z2):53-56. |
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| Authors: | LIANG Meng REN Ai-li Chen Shu-yi |
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| Institution: | Feed Quality Supervision and Testing Center（Ji nan）|Ministry of Agriculture|Ji nan 210036|China |
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| Abstract: | The anastrozole was extracted from compound feed by diethyl ether and dichloromethane.The extract was centrifuged and the supernatant was purified by MCX-SPE,then tested by HPLC.Quantitative analysis was carried out by UV-detector and qualitative analysis was by DAD.The result showed that the concentration of anastrozole and peak area was linear correlation between 0.1~1 000 μg/mL,r=0.998 9.The reclaim rate was 90%~112% with cofficient of variation less than 5%.The LDL of anastrozole was 1 μg/mL.Four medici... |
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| Keywords: | feed anastrozole HPLC |
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