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| 猪胞内劳森氏菌GXNN株4个抗原性候选基因抗原性分析 | |
| 引用本文： | 肖爱欢,谢丽华,廖承球,兰家暖,李茂宁,侯韶毅,陆仕勇,黄伟坚.猪胞内劳森氏菌GXNN株4个抗原性候选基因抗原性分析[J].广西农业科学,2010,41(1):62-65. |
| 作者姓名： | 肖爱欢 谢丽华 廖承球 兰家暖 李茂宁 侯韶毅 陆仕勇 黄伟坚 |
| 作者单位： | 1. 广西大学动物科学技术学院,南宁,530005 2. 梧州市动物疫病预防控制中心,广西捂州,543002 3. 广西农垦国有金光农场,南宁,530042 |
| 基金项目： | 广西科学研究与技术开发计划项目(桂科能0630006-6B) |
| 摘 要： | 参考GenBank上已发表的胞内劳森氏菌相关蛋白序列，设计了4对引物，分别扩增了4个抗原性候选基因（3个外膜蛋白基因和1个表面脂蛋白基因），并将4个抗原性候选基因构建好原核表达载体后进行原核表达、SDS—PAGE电泳和Western blotting分析。SDS—PAGE电泳检测初步确定重组融合蛋白pET32a—L10902、pET32a—L11022能够进行表达，分别得到53、37kDa的条带；Western blotting分析则初步确定pET32a—L11022具有抗原性。研究结果首次验证胞内劳森氏菌外膜蛋白基因是否具有抗原性，为诊断试剂盒及基因工程疫苗的研制提供理论基础。 |
| 关 键 词： | 胞内劳森氏菌 抗原性 候选基因 SDS—PAGECE泳 Western blotting 猪 |
Analysis of antigenicity in four antigenic candidate genes of Lawsonia intracellularis GXNN strain |
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| XIAO Ai-huan,XIE Li-hua,LIAO Cheng-qiu,LAN Jia-nuan,LI Mao-ning,HOU Shao-yi,LU Shi-yong,HUANG Wei-jian.Analysis of antigenicity in four antigenic candidate genes of Lawsonia intracellularis GXNN strain[J].Guangxi Agricultural Sciences,2010,41(1):62-65. | |
| Authors: | XIAO Ai-huan XIE Li-hua LIAO Cheng-qiu LAN Jia-nuan LI Mao-ning HOU Shao-yi LU Shi-yong HUANG Wei-jian |
| Institution: | 1 College of Animal Science and Technology;Guangxi University;Nanning 530005;China;2 Wuzhou Prevention and Control Center of Animal Epidemic Disease;Wuzhou;Guangxi 543002;3 Guangxi Jinguang State Farm;Nanning 530042;China |
| Abstract: | According to the associated protein sequences of Lawsonia intracellularis published in GenBank,four pairs of primers were designed and the genes of three outer membrane proteins and one ectal lipoprotein were amplified.After constructing the prokaryotic expression vectors for four antigenic candidate genes,the prokaryotic expression,SDS-PAGE electrophoresis and Western blotting analysis were performed.The results showed that two recombination fusion proteins pET32a-LI0902 and pET32a-LI1022 could be expresse... |
| Keywords: | Lawsonia intracellularis antigenicity candidate gene SDS-PAGE electrophoresis Western blotting |
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