| 亚硝酸盐对红螯光壳螯虾不同组织免疫相关酶活性及超微结构的影响 |
| |
| 引用本文： | 吕晓燕,李嘉尧,方燕,郭占林,赵云龙,李恺.亚硝酸盐对红螯光壳螯虾不同组织免疫相关酶活性及超微结构的影响[J].水产学报,2010,34(12):1812-1820. |
| |
| 作者姓名： | 吕晓燕 李嘉尧 方燕 郭占林 赵云龙 李恺 |
| |
| 作者单位： | 华东师范大学生命科学学院,上海,200062 |
| |
| 基金项目： | 上海市科委重大计划项目(08DZ1906401);上海市科委富民工程项目(09DZ1912900) |
| |
| 摘 要： | 应用透射电镜技术,结合生物酶测定,研究了水体中不同浓度亚硝酸盐胁迫下红螯光壳螯虾肝胰腺、鳃和肌肉组织中免疫相关酶的活性变化,以及对肝胰腺和鳃的形态学影响。结果显示,与对照组相比,亚硝酸盐胁迫下,3种组织的ACP、AKP、SOD以及GSH-PX的活性都显著降低(P<0.05);随着亚硝酸盐浓度增加,酶活力呈现降低的趋势;鳃组织Na+,K+-ATPase和Ca2+,Mg2+-ATPase的活性也显示出随亚硝酸盐浓度升高而降低的趋势。超微结构显示,随着亚硝酸盐浓度增加,鳃角质层受损、断裂;上皮细胞排列疏松、空泡化;细胞器变形;鳃腔内也出现空泡化现象,血细胞变形。肝胰腺上皮细胞排列杂乱无章,细胞裂解,空泡化;微绒毛受损、断裂,肝小管间距扩大、结缔组织变得稀薄,血细胞变形;高浓度组R细胞的脂滴减少,核膜解体,细胞膜破裂,空泡化加剧;F细胞的核糖体减少,空泡化加剧,内质网水肿。研究说明亚硝酸盐对红螯光壳螯虾3种组织的免疫相关酶活产生影响,并损伤肝胰腺和鳃的形态学结构,影响其生物学功能。
|
| 关 键 词： | 红螯光壳螯虾 亚硝酸盐 免疫相关酶 超微结构 |
| 收稿时间： | 8/5/2010 12:00:00 AM |
| 修稿时间： | 2010/9/16 0:00:00 |
Nitrite stress on immune related enzymes and the ultrastructure in different tissue of redclaw crayfish (Cherax quadricarinatus) |
| |
| LV Xiao-yan,LI Jia-yao,FANG Yan,GUO Zhan-lin,ZHAO Yun-long,LI Kai.Nitrite stress on immune related enzymes and the ultrastructure in different tissue of redclaw crayfish (Cherax quadricarinatus)[J].Journal of Fisheries of China,2010,34(12):1812-1820. |
| |
| Authors: | LV Xiao-yan LI Jia-yao FANG Yan GUO Zhan-lin ZHAO Yun-long LI Kai |
| |
| Institution: | LV Xiao-yan,LI Jia-yao,FANG Yan,GUO Zhan-lin,ZHAO Yun-long,LI Kai(Life Science College,East China Normal University,Shanghai 200062,China) |
| |
| Abstract: | The technique of transmission electron microscopy combined with enzymatic methods was used to analyse the variations of the immune-related enzymes activity in hepatopancreas,gills and muscles,and to compare the morphological changes of the hepatopancreas and gills of redclaw crayfish,Cherax quadricarinatus,under different concentrations of nitrite stress. The results showed that the activity of ACP,AKP,SOD and GSH-PX in three tissues under the stress of nitrite were significantly lower(P < 0. 05) compared w... |
| |
| Keywords: | Cherax quadricarinatus nitrite immune-related enzymes ultrastructure |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
| 点击此处可从《水产学报》浏览原始摘要信息 |
| 点击此处可从《水产学报》下载免费的PDF全文 |
|
