A strain-specific and a sex-associated STS marker for Asian arowana (Scleropages formosus, Osteoglossidae) |
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| Authors: | G H Yue D Ong C C Wong L C Lim & L Orban |
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| Institution: | Reproductive Genomics Group, Temasek Life Sciences Laboratory, Singapore; Ngee Ann Polytechnic, Singapore; Aquaculture Services Centre, Agri-Food &Veterinary Authority, Singapore; Department of Biological Sciences, The National University of Singapore, Singapore |
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| Abstract: | A sex‐associated amplified fragment length polymorphism and a strain‐specific random amplified polymorphic DNA marker were identified from Asian arowana (dragonfish; Scleropages formosus Müller & Schlegel) by screening pooled genomic DNA samples from three different strains as well as males and females respectively. Both markers were cloned, sequenced and successfully converted into sequence‐tagged‐site (STS) markers. The strain‐specific STS marker could be applied to differentiate the Indonesian golden strain of Asian arowana from the green and blood‐red strains before the stage when colours become identifiable. Individuals from the green strain could be sexed with an efficiency of 82.7% using the sex‐associated STS marker. Thus, populations with preferred sex ratios can be formed without the need of rearing a large number of fish. |
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| Keywords: | dragonfish bonytongue AFLP RAPD molecular sexing strain identification |
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