| 土壤链霉菌CaiF1抗菌物质的分离纯化及活性研究 |
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| 引用本文： | 杨辉,张茜,曾建民,李少华,谭志远.土壤链霉菌CaiF1抗菌物质的分离纯化及活性研究[J].安徽农业科学,2011,39(34):21037-21039. |
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| 作者姓名： | 杨辉 张茜 曾建民 李少华 谭志远 |
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| 作者单位： | 华南农业大学农学院,广东广州,510642 |
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| 基金项目： | 教育部跨世纪优秀人才支持计划项目 |
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| 摘 要： | 目的]从土壤链霉菌CaiF1发酵液中分离纯化抗菌物质,并研究该物质的活性。方法]采用乙酸乙酯萃取、大孔吸附树脂吸附、硅胶层析和制备型高效液相色谱（HPLC）等分离技术纯化抗菌物质,以金黄色葡萄球菌、白粉病菌为指示菌进行活性研究。结果]土壤链霉菌CaiF1发酵液中抗菌物质得到分离纯化;该物质在pH 2.0～10.0、100℃和紫外照射24 h条件下抑菌活性几乎不变,对白粉病的防治效果达69.7%。结论]分离纯化的土壤链霉菌CaiF1抗菌物质具有很好的稳定性,为其结构鉴定和开发应用提供理论依据。
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| 关 键 词： | 土壤链霉菌 抗菌物质 分离纯化 稳定性 |
Studies on Purification and Activity of Antibacterial Substances Derived from Soil Streptomyces sp.CaiF1 |
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| Institution: | YANG Hui et al(South China Agricultural University,Guangzhou,Guangdong 510642) |
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| Abstract: | Objective] Purification and activity of antibacterial substances derived from soil Streptomyces sp.CaiF1 were studied.Method] The antibacterial substances were separated and purified by Ethyl acetate extraction,macroporous adsorptive resin,silica gel chromatography and preparative high performance liquid chromatography(HPLC),taking Staphylococcus aureus and Powdery mildew as activity indicating bacterial.Result] Antibacterial substances were purified and the stability analysis of the extracts form Streptomyces CaiF1 fermentation broth showed stable at pH 2.0-10.0,100 ℃ and changed very little under UV treatment for 24 h.Inhibition rate of powdery mildew was 98.37%.Conclusion] The purified antibacterial substances showed good stability,which provided theoretical foundation for their structural identifications and future applications. |
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| Keywords: | Soil Streptomyces sp Antibacterial substances Separation and purification Stability |
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