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忍冬不同器官中绿原酸的提取及含量测定
引用本文:刘宝臣,李瑞国,侯文锐.忍冬不同器官中绿原酸的提取及含量测定[J].安徽农业科学,2011,39(32):19734-19735,19774.
作者姓名:刘宝臣  李瑞国  侯文锐
作者单位:泊头职业学院,河北泊头,062150;邢台学院生化系,河北邢台,054001;邢台市食品质量安全监督检验中心,河北邢台,054001
基金项目:河北省邢台市科技计划项目“金银花绿原酸提取及含量研究”(201032071)
摘    要:目的]提取忍冬不同器官中的绿原酸并进行含量测定。方法]采用酸乙醇回流法对"巨花一号"忍冬的藤、叶、花和花蕾中绿原酸进行提取,并采用高效液相色谱法对绿原酸进行检测和含量测定。结果]忍冬不同器官中绿原酸的含量不同,其中花和花蕾中绿原酸的含量较高,藤和叶中含量较少;其排序为:花(3.798 mg/g)〉花蕾(3.795 mg/g)〉藤(2.558 mg/g)〉叶(2.185 mg/g)。结论]从忍冬药材资源开发利用的角度而言,藤和叶也可以作为绿原酸提取和综合利用的材料。

关 键 词:忍冬(Lonicera  japonica  Thunb)  绿原酸  提取  测定

Extraction and Content Determination of Chlorogenic Acid in Different Organs of Lonicera japonica Thunb
Institution:LIU Bao-chen et al(Botou College,Botou,Hebei 062150)
Abstract:Objective] To extract the chlorogenic acid in different organs of Lonicera japonica Thunb.and then determine its content.Method] Chlorogenic acid in the vine,leaves,flowers and buds of "Giant Flower One" honeysuckles was extracted by acid ethanol method,and then determined by HPLC.Result] The content of chlorogenic acid was different in different organs of honeysuckles,it was higher in flowers and buds,while lower in vines and flowers;which was shown as flowers(3.798 mg/g)> buds(3.795 mg/g)> vines(2.558 mg/g)>leaves(2.185 mg/g).Conclusion] The veins and leaves of Lonicera japonica Thunb.could be used as the materials for developing medical resources,
Keywords:Lonicera japonica Thunb    Chlorogenic acid  Extraction  Determination
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