不同炮制方法对栀子中栀子苷含量的影响 |
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引用本文: | 杨海玲,覃葆,宋永龙,李勤,黄华艳,吴尤娇.不同炮制方法对栀子中栀子苷含量的影响[J].安徽农业科学,2011,39(32):19755-19757. |
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作者姓名: | 杨海玲 覃葆 宋永龙 李勤 黄华艳 吴尤娇 |
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作者单位: | 1. 广西中医学院药学院,广西南宁,530001 2. 江苏康缘药业股份有限公司,江苏连云港,222002 3. 广西玉林制药有限责任公司,广西玉林,537001 |
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摘 要: | 目的]比较栀子不同炮制品中栀子苷的含量。方法]采用HPLC法测定栀子中栀子苷的含量。色谱条件为:色谱柱为InertsilODS-2C18(250 mm×4.6 mm,5μm),流动相为乙腈-水(15∶85,V/V),流速为1.0 ml/min,检测波长为238 nm,柱温为40℃,进样量为20μl。结果]栀子不同炮制品中栀子苷的含量不同,其排序为生栀子(3.60%)〉炒炭品(3.36%)〉炒焦品(3.22%)〉炒焦品(轻,3.02%)〉炒黄品(2.77%)。结论]不同炮制方法对栀子中栀子苷含量有一定的影响。
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关 键 词: | 栀子(FRUCTUS GARDENIAE) 炮制 栀子苷 HPLC |
Effects of Different Processing Methods on the Geniposide Content in FRUCTUS GARDENIAE |
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Institution: | YANG Hai-ling et al(Guangxi Traditional Chinese Medical University,Nanning,Guangxi 530001) |
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Abstract: | Objective] The aim was to compare the effects of different processing methods on the geniposide content in FRUCTUS GARDENIAE.Method] Geniposide content in FRUCTUS GARDENIAE was determined by the method of HPLC.The chromatographic conditions were as follows: Inertsil ODS-2,C18(250 mm × 4.6 mm,5 μm) as the chromatographic column,acetonitrile-water(15∶ 85,V/V)as the mobile phase,1.0 ml/min flow rate,238 nm detection wavelength,40 ℃detection temperature,and 20 μl injection volume.Result] Geniposide content varied in different processed products of FRUCTUS GARDENIAE.Their contents were in the order of raw material(3.60%)>carbonized sample(3.36%)>parched sample(3.22%)>slightly brown-parched sample(3.02%) >yellow-parched sample(2.77%).Conclusion] Ddifferent processing methods had certain effects on the geniposide content in FRUCTUS GARDENIAE. |
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Keywords: | FRUCTUS GARDENIAE Processing method Geniposide HPLC |
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