| EUB338探针对真细菌细胞的检测灵敏性分析 |
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| 引用本文： | 王晓丹.EUB338探针对真细菌细胞的检测灵敏性分析[J].安徽农业科学,2011,39(33):20310-20311,20315. |
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| 作者姓名： | 王晓丹 |
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| 作者单位： | 上海市环境科学研究院,上海,200233 |
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| 基金项目： | 教育部留学回国人员科研启动基金资助项目，国家自然科学基金项目，上海市技术标准专项项目 |
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| 摘 要： | 目的]探讨EUB338探针对真菌的检测灵敏度。方法]以16株常见真菌为材料,使用荧光原位杂交法进行分析。结果]EUB338探针检测灵敏度的平均值为63.9%,改良方法可将检测灵敏度平均值提高到71.3%。结论]该研究可为从分子生物学角度检测土壤真菌提供科学依据。
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| 关 键 词： | EUB338 改良FISH 探针检测灵敏度 |
Analysis of the Sensitivity of Probe EUB338 for Enumerating Eubacterial Cells |
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| WANG Xiao-dan.Analysis of the Sensitivity of Probe EUB338 for Enumerating Eubacterial Cells[J].Journal of Anhui Agricultural Sciences,2011,39(33):20310-20311,20315. |
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| Authors: | WANG Xiao-dan |
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| Institution: | WANG Xiao-dan(Shanghai Academy of Environmental Science,Shanghai 200233) |
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| Abstract: | Objective] This paper aimed to improve the detection rate of probe EUB338 for eubacteria.Methods] Sixteen strains were used as materials for the experiment and analyzed through fluorescence in situ hybridization.Result] The average detection rate of probe EUB338 was 63.9%,which can be increased to 71.3% through modified FISH.Conclusion] This research was able to provide scientific basis for the detection of eubacteria in soil from the perspective of molecular biology. |
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| Keywords: | EUB338 Modified FISH Detection rate of probe |
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