Identification of SSR markers which could differentiate blast disease resistance accessions in finger millet (<Emphasis Type="Italic">Eleusine coracana</Emphasis> (L.) Gaertn.) |
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| Authors: | I N Bheema Lingeswara Reddy S Sivaramakrishnan |
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| Institution: | 1.Dept. of Agricultural Biotechnology,Acharya N.G. Ranga Agricultural University (ANGRAU),Rajendranagar, Hyderabad,India;2.Rajiv Gandhi Centre for Biotechnology,Poojappura, Thiruvananthapuram,India |
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| Abstract: | Microsatellites also known as SSR are the class of repetitive DNA sequences present throughout the genome of all eukaryotic organisms. The present study identified SSRs using biotinylated beads capture method. Ten sets of primers were designed based on sequences having at least (CT)10 repeats. A total of 27 accessions having a mix of both African (resistant) and Indian origin (resistant and susceptible) were assessed using 30 microsatellite markers. Amplification products were obtained for all 30 primers studied; 25 out of these primers were found to be polymorphic with 13 primers showing two alleles per locus. The current study identified markers which could differentiate between resistant and susceptible accessions and also segregate accessions based on geographical region. These informative SSR markers can be used in finger millet genetic improvement projects. |
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