| 稀有放线菌的选择性分离、鉴定及生物活性研究 |
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| 引用本文： | 刘最,李玉中,林慧敏,宋瑞洪.稀有放线菌的选择性分离、鉴定及生物活性研究[J].湖南农业科学,2016(10). |
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| 作者姓名： | 刘最 李玉中 林慧敏 宋瑞洪 |
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| 作者单位： | 衡阳师范学院生命科学与环境学院,湖南衡阳,421008 |
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| 基金项目： | 衡阳师范学院基金青年项目(14A04)；衡阳市科技局一般项目(2015KJ25) |
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| 摘 要： | 聚乳酸(PLA)和丝蛋白粉分别作为选择性碳、氮源可以有效提高稀有放线菌的分离效率,同时海洋环境中蕴藏着丰富的活性稀有放线菌,是发现新药的有效途径。为了筛选并鉴定海洋环境中产生活性物质的稀有放线菌,以聚乳酸和丝蛋白粉分别作为选择性碳、氮源,配制6种分离培养基,用平板稀释法分离放线菌;并用滤纸片法和MTT法对供测菌株发酵产物进行活性筛选。结果表明:共分离出96株稀有放线菌,分属于11个属;其中,80株菌对7种靶标菌显示出一定的抗菌活性;78株菌对肿瘤细胞具有不同程度的细胞毒活性。另外,比较了6种培养基对稀有放线菌的分离效果。从分离结果来看,最理想的是聚乳酸培养基。
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| 关 键 词： | 稀有放线菌 选择分离 聚乳酸(PLA) 丝蛋白粉 活性筛选 |
Selective Iolation and Identification of Rare Actinomycetes from Marine Habitats and Its bioactivity |
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| Abstract: | Poly-L-lactic acid and silk protein powder as selective carbon, nitrogen for isolation of rare actinomycetes is worth further researches. Marine habitats are rich in rare actinomycetes with bioactivity and may be an effective method of ifnding new medicine. This study was aimed to isolate and identify rare actinomycetes with bioactivity isolated from marine habitats. Rare actinomycetes were isolated using poly-L-lactic acid and silk protein as a selective carbon, nitrogen from six samples by agar plate dilution method. To test the biological active effects, we used paper-disk method with seven indicator organisms to detect the antimicrobial activity by its fermentation extracts, MTT assay with 2 human tumor cell lines to monitor anti-tumor activitiy. Among of them, by eliminating the same strains from one genus and then 120 strains were identiifed by 16SrRNA gene sequence analysis, 96 strains were classiifed into 11 genera besides Streptomyces. 80 strains displayed anti-microbial activities. Meanwhile, 78 strains displayed anti-tumor activities. Six types of culture medium for isolating rare actinomycetes had been compared, and results revealed that poly-L-lactic acid culture medium was the best for isolation. |
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| Keywords: | rare actinomycetes selective isolation poly-L-lactic acid silk protein bioactivity screening |
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