| 侧孢芽孢杆菌C5发酵条件及培养基的优化 |
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| 引用本文： | 吴迎奔,陈薇,贺月林,许丽娟,王震,汪彬.侧孢芽孢杆菌C5发酵条件及培养基的优化[J].湖南农业科学,2016(9):4-6. |
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| 作者姓名： | 吴迎奔 陈薇 贺月林 许丽娟 王震 汪彬 |
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| 作者单位： | 湖南省微生物研究院,湖南 长沙,410009 |
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| 基金项目： | 湖南省科技计划(2015CK3008)；湖南省生猪产业技术体系生猪产业规模养殖与环境控制岗位项目 |
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| 摘 要： | 为了更加有效地利用侧孢芽孢杆菌C5,研究了该菌株的生长特性,并对其发酵条件及培养基进行了优化。结果表明,侧孢芽孢杆菌C5的生长规律为:0~4h延滞期,4~16h指数期,16h后进入稳定期;最佳发酵条件为:初始pH值7.0、培养温度30℃、接种量3%~5%、转速180r/min;最适培养基为:葡萄糖20.0g/L、酵母膏10.0g/L、MnSO_4 0.2g/L、CaCl_2 0.3g/L、KH_2PO_4 0.1g/L、MgSO_4 0.05g/L。在上述条件下,侧孢芽孢杆菌C5的发酵芽孢数可达1.08×109CFU/mL。
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| 关 键 词： | 侧孢芽孢杆菌 发酵 条件 培养基 优化 |
Optimizing Fermentation Condition for Bacillus laterosporus C5 |
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| Abstract: | In order to use Bacillus laterosporus C5 efficiently in production, the growth characteristics were studied, and the liquid fermentation medium and fermentation conditions were optimized. The results showed that the optimal fermentation conditions were initial pH 7.0, temperature 30 ℃ , inoculation rate 3%-5%, and rotate speed 180 r/min; the optimum medium was glucose 20.0 g/L, yeast extract 10.0 g/L, MnSO4 0.2 g/L, CaCl2 0.3 g/L, KH2PO4 0.1 g/L, MgSO4 0.05 g/L. Under the conditions, the spore yield with optimum medium formula was up to 1.08×109 CFU/mL. |
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| Keywords: | Bacillus laterosporus fermentation conditions condition culture medium orthogonal optimization |
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