| RP-HPLC法测定迷迭香药材中鼠尾草酸 |
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| 引用本文： | 李海,李鹏辉,黄惠华,李佳银.RP-HPLC法测定迷迭香药材中鼠尾草酸[J].湖南农业科学,2016(10). |
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| 作者姓名： | 李海 李鹏辉 黄惠华 李佳银 |
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| 作者单位： | 1. 华南理工大学,广东广州,510641;2. 湖南农业大学,湖南长沙,410128;3. 湖南三福生物科技有限公司,湖南长沙,410100 |
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| 摘 要： | 为建立高效液相色谱法测定迷迭香中鼠尾草酸含量的方法,以乙腈-0.1%磷酸水(60∶40)为流动相,检测波长为230 nm;柱温30℃,等度洗脱,流速为1.0 m L/min。结果显示,鼠尾草酸在0.277 2~1.016 4 mg/m L范围具有良好的线性关系(R2=0.999 92),回收率为97.56%。该方法快速、灵敏、准确,目标峰分离度良好,可为迷迭香药材以及提取物的质量控制提供依据。
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| 关 键 词： | 高效液相色谱 迷迭香 鼠尾草酸 |
Determination of Carnosic Acid in Rosemary by RP-HPLC |
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| Abstract: | To establish a HPLC method for the determination of carnosic acid in Rosemarinus ofifcimalis L., the anaiysis was carried out on Pheromenex Ecosil C18 column (200 mm ×4.6 mm,5 μm), the mobile phase was composed of actonitrile and 0.1% phosphoric acid aqucous (60∶40, v/v).The detection wavelength was 210 nm, with lfow rate 1.0 mL/min at column temperature of 30℃.The standard curve of linear relationship was of 0.277 2-1.016 4 mg/mL and R2=0.999 92, average recovery was 97.56%. The method was accurate, sensitive, simple and reliable for the determination of carnosic acid in Rosemarinus oifcinalis L., suitable for the quality evaluation of Rosemarinus ofifcinalis L. |
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| Keywords: | HPLC Rosemarinus officinalis L carnosic acid |
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